二棱大麦LEA cDNA的克隆与测序  

Cloning of a group 3 Lea cDNA from tworow barley

作　　者：郭卫东[1] 饶景萍[1] 李嘉瑞[1] 郑学勤[2] 

机构地区：[1]西北农林科技大学园艺系,陕西杨陵712100 [2]中国热带农业科学院生物技术国家重点实验室,海南儋州571737

出　　处：《西北农业大学学报》2000年第2期8-12,共5页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

基　　金：杨陵农业科技开发基金资助项目! ( 41 3 0 2 0 2 )

摘　　要：从二棱大麦 ( H ordeum distichon Linn.)幼苗中提取总 RNA,通过 RT- PCR获得目的片段 HDCS1 ,并采用 T-载体进行克隆。测序结果表明 ,HDCS1全长 664bp,含有 1个完整的阅读框架。其编码蛋白质全长 2 0 2个氨基酸 ,含有 8个由 1 1个氨基酸残基组成的基元序列 ,属第 3组 LEA蛋白。HDCS1与大麦 ( H ordeum vulgare L.) L EA蛋白、HVA1具有高度同源性 ,其差异在于 HDCS1比 HVA1缺少一个完整的由 1Total RNA is extracted from tworow barley ( Hordeum distichon Linn.) seedlings induced with 0.1 mmol/L ABA and reversely transcribed to first strands of cDNA.The interest fragment,HDCS1,is obtained by PCR and cloned by T overhang vectors.HDCS1 is 664 bp long containing one open reading frame.It encodes a deduced protein of 202 amino acid residues,which contains eight motifs composed of 11 amino acids and belongs to group 3 LEA protein.HDCS1 is highly homologous to barley ( H.vulgare Linn.) HVA1.The only difference between them is that the number of 11 mer repeating units of HDCS1 is one less than that of HVA1.

关 键 词：二棱大麦 LEA基因 克隆 序列分析 抗旱基因工程 

分 类 号：S512.303.4[农业科学—作物学] S332.4