RP-HPLC法同时测定芎菊上清丸中绿原酸、盐酸小檗碱和黄芩苷的含量  

RP- HPLC simultaneous determination of chlorogenic acid,berberine hydrochloride and baicalin contents in Xiongju Shangqing pills

作　　者：郝乘仪[1] 郭淑英[1] 冯波[1] 崔佰吉[1] 

机构地区：[1]吉林医药学院药学院,吉林132013

出　　处：《药物分析杂志》2014年第1期193-197,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立RP—HPLC法同时测定芎菊上清丸中绿原酸、盐酸小檗碱和黄芩苷的含量。方法：采用DIKMA Platisil ODS色谱柱（250mm×4．6mm，5μm），柱温30℃，以甲醇（A）-0．1％磷酸水溶液（B）为流动相，梯度洗脱（0～5min，40％A；5～20min．40％A→60％A；20～30min，60％A），流速1mL·min^-1，变换波长检测（327nm，绿原酸；345nm，小檗碱；278nm，黄芩苷）。结果：绿原酸、盐酸小檗碱和黄芩苷的线性范围分别为0．11—2．2μg（r=0．9997）、0．021～0．42μg（r=0．9999）、0．03～0．6μg（r=0．9998），平均回收率在98．45％和99．07％之间。结论：该法检测效率高，专属性强，重复性好，可用于芎菊上清丸中绿原酸、盐酸小檗碱和黄芩苷的含量测定及其质量控制。Objective： To establish a RP - HPLC method for simultaneous determination of chlorogenic acid, berberine hydrocbloride and baicalin in Xiongju Shangqing pills. Methods： The separation was performed on a DIK- MA Platisil ODS column （250 mm×4.6mm,5μm） with gradient elution （0 -5 min ,40% A ;5 -20 min ,40% A→60% A ;20 -30 min ,60% A） using methanol（A） and 0.1% phosphoric acid aqueous solution（B） as mobile phase at a flow rate of 1 mL · min^-1. The detection wavelengths were 327 nm for chlorogenic acid ,345 nm for berberine and 278 nm for baicalin, respectively. Results： Linear ranges of chlorogenic acid, berberine hydrochloride and baicalin concentrations were 0.11 - 2.2 μg （ r = 0. 9997 ） , 0. 021 - 0.42 μg （ r = 0. 9999 ） , 0.03 - 0.6μg （ r = 0. 9998 ） ,respectively. Their average recoveries fell between 98.45% and 99.07%. Conclusion： The RP - HPLC method established can be used to determine the contents of chlorogenic acid,berberine hydrochloride and baicalin in Xiongju Shangqing pills,which may also be applied as a standard for the quality control of Xiongju Shangqing pills.

关 键 词：反相高效液相色谱法 芎菊上清丸 绿原酸 小檗碱 黄芩苷 中成药含量测定 

分 类 号：R917[医药卫生—药物分析学]