芦荟全叶冻干粉的一般药理学实验研究  

General Pharmacological Study of Aloe's Whole-leaf Freeze-dried Powder

作　　者：杨海润[1] 陈会丛[1] 朱晓光[1] 张广平[1] 翟建英[1] 张思玉[1] 蒋威[1] 李晋生[1] 解素花 

机构地区：[1]北京中研同仁堂医药研发有限公司,中药复方新药开发国家工程研究中心,北京100079 [2]北京同仁堂科技发展股份有限公司,北京100079

出　　处：《中国中医药信息杂志》2014年第1期54-57,61,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家高技术研究发展计划(2010AA023006)

摘　　要：目的观察芦荟全叶冻干粉对实验动物心血管系统、中枢神经系统和呼吸系统的影响,探讨其一般药理作用,为临床用药提供实验依据。方法将48只小鼠随机分为空白组和芦荟全叶冻干粉高、中、低剂量组,每组12只。给药组分别按12.20、3.90、0.65 g原药材/kg剂量灌胃给药,1次/d,空白组灌胃等体积灭菌蒸馏水。连续给药3 d,观察该药对小鼠自主活动行为和小鼠协调运动及对戊巴比妥钠阈下剂量致小鼠催眠和阈上剂量致小鼠睡眠时间的影响;比格犬24只分组同上,每组6只。给药组分别按6.10、3.41、0.71原药材/kg剂量十二指肠灌胃给药,1次/d,空白组灌胃等体积灭菌蒸馏水,记录麻醉犬给药后30、60、120、150 min的潮气量和呼吸频率、收缩压、舒张压、平均动脉压、心率、心电图。结果芦荟全叶冻干粉高、中、低剂量组对小鼠一般行为、自发活动、协调运动、戊巴比妥钠阈下剂量催眠只数和阈上剂量睡眠时间无明显影响,但高剂量对戊巴比妥钠睡眠的潜伏期有影响(P<0.01);给药组对麻醉犬的潮气量和呼吸频率、收缩压、舒张压、平均动脉压、心率、心电图各项生理指标无显著影响。结论芦荟全叶冻干粉在实验剂量下,对实验动物心血管系统和呼吸系统无明显影响,但对中枢神经系统的影响还有待进一步验证。Objective To study the general pharmacological effects of Aloe＇s whole-leaf freeze-dried powder （AWFD）, and observe its influence on cardiovascular system, nervous system and respiratory system of laboratory animals, so as to offer an experimental basis for clinical application. Methods Forty-eight mice were randomized into blank control group, high dosage group, medium dosage group and low dosage group of AWFD （12 mice for each group）. AWFD high, medium and low dosage groups were treated by intragastric at the dose of 12.20, 3.90, 0.65 g/（kg？d）, blank control group was treated by equal volume of sterilized distilled water. After three days, general behavior, spontaneous activity, coordinated movement, sleep situation induced by sodium pentobarbital in subthreshold dose and suprathreshold dose were observed. Twenty-four beagle were randomized into blank control group, high dosage group, medium dosage group and low dosage group of AWFD （6 beagles for each group）. AWFD high, medium and low dosage groups were treated by duodenum at the dose of 6.10, 3.41, 0.71 g/（kg？d）, blank control group was treated by equal volume of sterilized distilled water. The influence on blood pressure, heart rate, electrocardiogram, breathing flow and frequency in anesthetic dogs were observed. Results Three dosages of AWFD had no obvious influence on spontaneous activity and coordinated movement in mice, and had no evidently influence on sleep number and duration, but the high dosage group of AWFD had influence on sleep latency （P〈0.01）. AWFD had no impact on blood pressure, heart rate, electrocardiogram, breathing flow and frequency in anesthetic dogs. Conclusion AWFD has no evident effects on cardiovascular system and respiratory system in laboratory animal, however, the impact on the central nervous system remains to be further verified.

关 键 词：芦荟全叶冻干粉 中药药理学 小鼠 犬 

分 类 号：R285.5[医药卫生—中药学]