寿胎丸对复发性流产小鼠蜕膜组织α-烯醇化酶表达的影响  

Effect of Shoutaiwan on Expression of α-enolase in Decidua Tissue of Recurrent Abortion Mice

作　　者：谭展望[1] 雷磊[1] 李慧芳[1] 朱伟[1] 孟琼[1] 罗蕾[1] 卢丽丽[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《中国中医药信息杂志》2014年第1期65-68,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(30973771);教育部博士点基金博士导师项目(20094323110004)

摘　　要：目的通过观察寿胎丸对复发性流产小鼠蜕膜组织α-烯醇化酶表达的影响,探讨其安胎的作用机制。方法 CBA/J×BALB/C建立正常妊娠模型,CBA/J×DBA/2建立复发性流产模型,将复发性流产模型CBA/J×DBA/2孕鼠按怀孕先后顺序随机分为模型组及寿胎丸高、中、低剂量组,从妊娠第1日开始灌胃给药,至孕14 d处死小鼠,采用免疫组化与蛋白印迹方法检测各组蜕膜组织α-烯醇化酶的表达。结果小鼠模型组与正常组比较,α-烯醇化酶表达明显升高,差异有统计学意义(P<0.01);与模型组比较,寿胎丸高、中、低剂量组α-烯醇化酶表达均降低,差异有统计学意义(P<0.01);寿胎丸高剂量组α-烯醇化酶表达与中、低剂量组之间差异无统计学意义(P>0.05)。结论寿胎丸通过下调复发性流产小鼠蜕膜组织α-烯醇化酶的表达,从而实现维持妊娠,这可能是其安胎的作用机制之一。Objective To observe the effect of Shoutaiwan on the expression ofα-enolase in the decidua tissue of recurrent abortion mice. Methods The abortion-prone CBA/J × DBA/2 matings were established as the model of recurrent abortion and the nonabortion-prone CBA/J×BALB/C matings were used as the model of normal pregnancy. The model of recurrent abortion CBA/J × DBA/2 of pregnant mice were randomly divided into model group and Shoutaiwan high-, medium-, low-dose groups, pregnant mice of every group were orally administrated in different doses. On the 14th day of pregnancy, the mice were killed. The expression ofα-enolase was detected by using immunohistochemical method and Western Blot. Results α-enolase expression in the model group was significantly higher than the normal pregnancy group （P〈0.01）. Compared with the model group, Shoutaiwan low-, medium- and high-dose group significantly decreasedα-enolase expression of pregnant mice （P 〈0.01）. Compared with high-dose group, Shoutaiwan medium-and low-dose group showed no significant difference （P〉0.05）. Conclusion Shoutaiwan could down-regulate the expression ofα-enolase in the decidua tissues of recurrent abortion mice, which may be one of its mechanisms of preventing miscarriage.

关 键 词：寿胎丸 复发性流产 蜕膜组织 α-烯醇化酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]