HPLC测定增液承气口服液中梓醇含量  

Content Determination of Catalpol in Zengye Chengqi Syrupus by HPLC

作　　者：王勤 袁小龙[2] 

机构地区：[1]张掖市食品药品检验所,甘肃张掖734000 [2]甘肃省张掖医学高等专科学校,甘肃张掖734000

出　　处：《中国中医药信息杂志》2014年第1期83-84,87,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的：建立增液承气口服液中梓醇的含量测定方法。方法采用高效液相色谱法,色谱柱为Eclipse XDB C18（150 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸溶液（1∶99）,检测波长为210 nm,柱温为30℃,流速为1.0 mL/min。结果梓醇对照品进样量在0.052～0.258μg（r＝0.9999）范围内与峰面积积分值呈良好的线性关系,平均加样回收率为98.23%（RSD＝0.76%,n＝9）。结论所建立的定量方法准确、重复性好、专属性强,可作为控制和评价本品质量的方法。Objective To establish a method for the determination of catalpol in Zengye Chengqi Syrupus by HPLC. Methods High performance liquid chromatography was performed on an Eclipse XDB C18 column （150 mm×4.6 mm, 5 μm） with acetonitrile-0.1%phosphoric acid （1∶99） as the mobile phase at a flow rate of 1.0 mL/min, 210 nm as the detection wavelength, the temperature of column was set at 30 ℃. Results Catalpol showed good linear relationship at the range of 0.052-0.258 μg （r=0.999 9）, the average recovery （n=5） was 98.23% （RSD=0.76%, n=9）. Conclusion The method was accurate, reliable and specific. It can be used for the quality control and evaluation of Zengye Chengqi Syrupus.

关 键 词：增液承气口服液 梓醇 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]