人类免疫缺陷病毒感染儿童抗病毒治疗后外周血单个核细胞线粒体DNA含量检测  

Mitochondrial DNA of peripheral blood mononuclear cells detection in HIV-infected children treated with anti-retroviral therapy

作　　者：张洪海[1] 张蕾[2] 孙玉[1] 石英 郑加生[1] 陈德喜 

机构地区：[1]首都医科大学附属北京佑安医院肿瘤微创介入中心,北京100069 [2]滨州医学院附属医院感染性疾病科,山东滨州256610 [3]北京市肝病研究所,北京100069

出　　处：《临床荟萃》2014年第1期17-20,共4页Clinical Focus

基　　金：国家"十二五"重大专项(2012BAI15B08;2012ZX10001004-002);国家自然科学基金资助项目(81100288)

摘　　要：目的研究我国人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染儿童抗逆转录病毒治疗(active anti-retrovirus therapy,ART)后外周血单个核细胞(peripheral blood mononuclear cells,PBMC)线粒体DNA含量及其相关影响因素。方法选择96例ART的HIV感染患儿、30例未治疗的HIV感染患儿及55例正常对照儿童,检测PBMC线粒体DNA含量,结合儿童的临床信息及实验室检测结果进行综合分析。结果 ART组患儿PBMC内线粒体DNA含量为(17.39±9.31),明显低于未治疗组患儿(26.35±11.74)及对照组儿童(28.37±12.14)PBMC内线粒体DNA含量,差异有统计学意义(P<0.01);患儿ART后PBMC内线粒体DNA含量与基线CD4+T淋巴细胞计数、基线病毒载量、性别、年龄及HIV感染途径均无相关性。结论 PBMC线粒体DNA含量可反映儿童抗病毒治疗后的线粒体损伤毒性,可能成为线粒体毒性检测指标。Objective To investigate the mitochondrial DNA contents in peripheral blood mononuclear cells （PBMC） and its related influencing factors in human immunodeficiency virus（HIV） infected children who were treated with anti-retrovirus therapy（ART）. Methods The study included 96 HIV infected children who were treated with ART,30 HIV infected children who were not treated with ART（naive） and 55 control children were chosen to detect mitochondrial DNA contents in PBMC. The clinical information and laboratory test results of the children were calculated to obtain comprehensive analysis. Results Mitochondrial DNA contents in PBMC of ART-treated children （17.39±9.31） were lower than that of naive （28.37 ± 12.14） and control children （26.35±11.74） （ P 〈0.01）. Mitochondrial DNA contents in PBMC of ART treatment were not statistically related with baseline CD4＋ T cell number,baseline viral load, gender, age, and route of HIV transmission. Conclusion Mitochondrial DNA contents in PBMC could reflect the mitochondrial toxicity of ART treated children and may be a marker of mitochondrial toxicity test.

关 键 词：获得性免疫缺陷综合征 抗逆转录病毒治疗 高效 DNA 线粒体 线粒体毒性 儿童 

分 类 号：R512.91[医药卫生—内科学] R453[医药卫生—临床医学]