正铁血红素改善链脲佐菌素诱导的糖尿病大鼠肝损伤  

Protective effects of hemin against hepatic tissue injury in streptozotocin-induced diabetic rats

作　　者：邢邯英[1] 刘敏[1] 野战鹰[1] 张冬会[1] 

机构地区：[1]河北省人民医院老年医学重点实验室,河北石家庄050051

出　　处：《临床荟萃》2014年第1期56-58,共3页Clinical Focus

摘　　要：目的探讨血红素加氧酶1(HO-1)诱导剂正铁血红素和抑制剂锌原卟啉对糖尿病大鼠肝功能的影响及相关机制。方法以链脲佐菌素腹腔注射诱导糖尿病SD大鼠模型,大鼠分为对照组、糖尿病组、正铁血红素组和锌原卟啉组。应用试剂盒检测各组大鼠血清游离脂肪酸(FFA)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性,肝组织匀浆总抗氧化能力(TAOC)和丙二醛(MDA);逆转录-聚合酶链反应(RT-PCR)法检测肝脏组织白细胞介素1(IL-1)和肿瘤坏死因子α(TNF-α)mRNA表达水平。结果与对照组比较,糖尿病组大鼠血清AST、ALT、肝组织MDA、IL-1、TNF-αmRNA水平均明显增高(P<0.01或<0.05),分别是(91.59±12.38)U/L vs(50.19±12.65)U/L、(45.64±9.68)U/L vs(15.55±7.79)U/L,(0.81±0.22)nmol/mg vs(0.50±0.08)nmol/mg、12.32±3.51vs 7.02±1.99、22.24±4.48vs 10.54±2.36;TAOC下降(P<0.05);与糖尿病组大鼠比较,正铁血红素组大鼠ALT、TNF-α表达水平明显下降,TAOC增高(P<0.05或<0.01);锌原卟啉组大鼠较糖尿病组大鼠FFA、ALT、AST、MDA均有明显上升(P<0.05或<0.01),而TAOC下降(P<0.05)。结论 HO-1诱导剂正铁血红素可改善糖尿病大鼠肝损伤,而其抑制剂则加重肝脏损伤。Objective To explore the effect of hemin, an inducer of heme oxygenase-1 （HO-1）, and zinc protoporphyrin （an inhibitor of HO-1） On the oxidative injury in the liver in diabetic rats. Methods Diabetes were induced by intraperitoneal injection of streptozotocin. The rats were divided into four groups： control group, diabetes mellitus group,hemin group and zinc protoporphyrin group. Five weeks after streptozotocin injection, free fatty acids （FFA） in serum and total antioxidant capacities（TAOC）, malondialdehyde contents（MDA） in hepatic tissue were measured by using kits. The expression of IL-1 and TNF-α in hepatic tissue were examined by reverse transcription polymerase（RT-PCR）. Results Compared with control group,AST（91. 59±12.38） U/L vs （50.19±12.65） U/L, ALT（45. 64±9. 68） U/L vs （15.55±7.79） U/L in serum,MDA（0. 81±0. 22） nmol/mg vs （0.50±0.08） nmol/mg, IL-1 12.32±3.51 vs 7.02±1.99 and TNF-α mRNA 22.24±4.48 vs 10.54±2.36 in hepatic tissue of diabetic rats were increased（ P 〈0.01 or 〈0.05） ,while TAOC was decreased（ P 〈0.05）. ALT and TNF-α expressions in hemin group were lower than those in diabetes mellitus group（ P 〈0.05） ,while TAOC was elevated in liver（ P 〈0.01）. Compared to those of diabetes mellitus group, FFA, ALT, AST,MDA expressions in zinc protoporphyrin group were elevated significantly（ P 〈0.01 or 〈0.05） ,while TAOC was lower in liver（ P 〈0.05）. Conclusion Upregulation of HOt.1 gene expression by heroin may improve hepatic tissue injury in diabetic rats, while down-regulation of heme oxygenase-1 by zinc protoporphyrin could aggravate hepatic injury.

关 键 词：糖尿病 血红素加氧酶-1 血红素 肝脏 氧化应激 

分 类 号：R587.1[医药卫生—内分泌]