多孔钛/间充质干细胞/富血小板血浆修复骨缺损  

The repair of bone defect with porous titanium/marrow mesenchymal stem cells/platelet-rich plasma implantation

作　　者：舒涛[1] 项舟[1] 杨婧[1] 高宇[1] 高博[1] 段鑫[1] 郑晓佐[1] 范红松 朱向东 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]四JlI大学国家生物材料工程技术研究中心

出　　处：《中华实验外科杂志》2014年第1期129-131,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（31370984）

摘　　要：目的 制备多孔钛/间充质干细胞（MSCs）/富血小板血浆（PRP）材料,探讨其对兔股骨远端骨缺损修复.方法 多孔钛、成骨诱导MSCs、PRP复合成多孔钛/MSCs/PRP和多孔钛/MSCs,体外分别培养4、9d,扫描电镜观察.新西兰大白兔随机分成4组：A组：多孔钛移植；B组：多孔钛/MSCs复合物移植；C组：多孔钛/MSCs/PRP复合物移植；空白组：未填充材料.术后6周行大体观察、甲苯胺蓝染色、Micro-CT检测和生物力学实验.结果 体外培养4、9d,电镜下多孔钛/MSCs/PRP组微孔内MSCs数量比多孔钛/MSCs组多.动物实验术后6周材料孔隙内骨组织生长,新生骨量（NBV,％）：A组：4.66±3.01,B组：10.08±2.55,C组：15.40±3.71,差异均有统计学意义（P＜0.05）.结合强度：A组：（1.49±0.55） mPa,B组：（3.29 ±0.55） mPa,C组：（5.31±1.01） mPa,差异均有统计学意义（P＜0.05）.结论 多孔钛/MSCs/PRP材料具有良好生物相容性,对骨缺损修复效果良好.Objective To repair the bone defect of the distal femur in rabbits by using bone marrow mesenchymal stem cells （MSCs） and platelet-rich plasma （PRP） into a porous titanium scaffold.Methods Porous titanium/MSCs composite and porous titanium/MSCs/PRP composite were cultured in vitro for 4 and 9 days.The growth of MSCs on the composites was examined by scanning electron microscope （SEM）.4..0 mm ＆#215; 4.0 mm ＆#215; 3.5 mm bone defects of the distal femur was created bilaterally.Porous titanium （group A）,porous titanium/MSCs composite （group B）,porous titanium/MSCs/PRP composite （group C） were transplanted into the untreated femoral defects as blank control group.At 6th week after operation,the samples were observed by micro-CT,histology,histomorphometric analyses and biomechanical testing.Results MSCs adhered to the scaffolds after culture for 4 days in vitro.More MSCs adhered to the scaffolds of porous titanium/MSCs/PRP composite than to those of porous titanium/MSCs composite.In vivo,bone formation and integrity into implant were observed in groups A,B and C after 6 weeks.New bone volume （NBV,%） in group B （10.08 ±2.55） and group C （15.40 ±3.71） was significantly greater than in group A （4.66 ±3.01） （P 〈0.05）,and that in group C greater than in group B （P 〈0.05）.Biomechanical testing indicated that the bone bonding strength in group C （5.31 ± 1.01） was significantly stronger than in group A （1.49 ± 0.55 mPa） and group B （3.29 ± 0.55 mPa） （P 〈 0.05）,and that in group B stronger than in group A （P 〈 0.05）.Conclusion Porous titanium/MSCs/PRP composite exhibits a favorable osteogenesis,biocomPatibility in the repair of femoral defect,and can be used as a substitute of bone tissue engineering material.

关 键 词：骨缺损 组织工程 钛 富血小板血浆 间充质干细胞 

分 类 号：R318.08[医药卫生—生物医学工程]