花生废弃物中白藜芦醇和白藜芦醇苷的提取与分析  

Extraction,Identification and Content Analysis of Resveratrol and Polydatin in Peanut Waste Materials

作　　者：韩寒冰[1] 沈兰[2] 李海航[2] 

机构地区：[1]广东石油化工学院化学与生命科学学院,茂名525000 [2]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州510631

出　　处：《华南师范大学学报（自然科学版）》2014年第1期46-50,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省科技计划项目(2011B020310008);广东省自然科学基金项目(S201101000368)

摘　　要：采用溶剂浸泡提取法从珍珠红14、粤油114、汕油523和湛油75花生品种的废弃物中提取白藜芦醇和白藜芦醇苷,根据提取物的HPLC出峰时间及与对照品的相加试验,证实在花生品种的不同器官中均存在白藜芦醇和白藜芦醇苷.不同花生品种和同一品种不同器官中2种活性物质的含量差异很大;在珍珠红14的各器官中含量最高,湛油75中含量最低.各品种花生根中2种化合物的含量最高.珍珠红14的根、茎、叶和果壳中白藜芦醇含量分别为148.7、33.3、23.1、9.4μg/g,白藜芦醇苷含量分别为133.7,37.7,27.7、11.5μg/g.珍珠红14的根中白藜芦醇和白藜芦醇苷含量高于葡萄和桑椹等材料.该研究结果为花生废弃物的综合利用提供了依据.Large amount of plant waste materials （ root, stem, leaf and fruit shell） are produced in peanut （Arachis hypogaea） cultivation and processing. We investigated the extraction, identification and contents of resveratrol and polydatin in different organ wastes of four varieties of peanut, Pearl Red 14, Guangdong Oil 114, Santou Oil 523 and Zhanjian Oil 75. The optimal extraction is that dried material was crushed and sieved through a 40 mesh sieve, ultrasonic-extracted with 60% ethanol for 3 times. Contents of the bioactive substances were analyzed by HPLC. Both resverstrol and polydatin were identified in all root, stem, leaf and fruit shell of the four varieties of peanut. The contents of the two compounds varied greatly in different peanut varieties and organs, with the highest contents for both compounds in Pearl Red 14, and the lowest in Zhanjian Oil 75. The root of all varieties contained much higher resveratrol and polyatin than other organs. Resveratrol contents were 148.7, 33.3, 23.1 and 9.4 μg/g, and polyatin contents were 133.7, 37.7, 27.7 and ll. 5 μg/g in Pearl Red 14 roots, stems, leaves and shell, re- spectively. The contents of both resveratrol and polydatin in Pearl Red 14 root are much higher than in grapes, mulberries and other plant materials. The work provides useful information for comprehensive utilization of peanut wastes.

关 键 词：白藜芦醇 白藜芦醇苷 花生废弃物 HPLC 

分 类 号：R286[医药卫生—中药学]