三氧化二砷对人脑胶质瘤SHG44细胞增殖的抑制作用  

Inhibition Effects of Arsenic Trioxide on SHG44 Human Glioma Cells

作　　者：黄辉[1] 蒋宏玲[2] 刘芬菊[3] 白雪[1] 何卓凯[1] 蔡锐[1] 张荣君[1] 赵博[1] 刘美莲[1] 

机构地区：[1]桂林医学院附属医院放疗科,桂林541001 [2]桂林医学院附属医院康复科,桂林541001 [3]苏州大学放射医学与公共卫生学院,苏州215007

出　　处：《医药导报》2014年第1期34-38,共5页Herald of Medicine

基　　金：广西卫生厅自筹经费科研课题基金资助项目(Z2011185)

摘　　要：目的探讨三氧化二砷(As2O3)对人脑胶质瘤SHG44细胞增殖的抑制作用及其作用机制。方法采用噻唑蓝(MTT)法观察As2O3对SHG44细胞的增殖抑制率;激光共聚焦显微镜检测As2O3作用于SHG44细胞后细胞内活性氧自由基(ROS)水平变化。结果 As2O3对SHG44细胞增殖具有明显抑制作用,随着As2O3浓度的升高和作用时间的延长,细胞的增殖抑制率升高,12μmol·L-1As2O3对SHG44细胞增殖的抑制率可达63.8%;As2O3可以明显提高SHG44细胞内ROS水平,其ROS水平随As2O3作用浓度的增高明显增高,具有浓度依赖关系。结论 As2O3能明显抑制SHG44细胞的增殖,抑制作用呈现剂量依赖性和时间依赖性,其机制与升高细胞内活性氧水平有关。Objective To investigate the anti-proliferation effects of arsenic trioxide（As203） on human glioma cells-44 （SHG44） in vitro and its mechanism. Methods The inhibitory ratio of the cells by As2O3 was measured by MTT assay. The intracellular ROS were determined by a probe （DCFH-DA） under a laser scanning confoeal microscopy. ResultsAs2O3 significantly inhibited SHG44 cells proliferation. The proliferation inhibition rate was dose-dependent and time-dependent. The proliferation inhibition by 12 iAmol ·L-1 As2O3 on SHG44 cells was as high as 63. 8%. After treatment with As2O3, the intracellular ROS was increased markedly in a concentration-dependent manner. Conclusion As2O3 can inhibit the proliferation of SHG44 cells in both dose-dependent and time-dependent manners. The inhibition was associated with elevation of intracellular ROS .

关 键 词：三氧化二砷 胶质瘤 SHG44细胞 抑制作用 

分 类 号：R979.1[医药卫生—药品] R969[医药卫生—药学]