消瘀片对粥样硬化兔血管壁组织中ET、NO及细胞凋亡的影响  

Effects of Xiaoyu tablets on ET,NO and apoptotic cells of atherosclerotic vessel wall in rabbits

作　　者：谢梅林[1] 顾振纶[1] 陈可冀[2] 周文轩[3] 郭次仪 

机构地区：[1]苏州中药研究所,苏州215007 [2]中国中医研究院,北京100091 [3]中国香港香港保健协会

出　　处：《中国现代应用药学》2000年第6期434-436,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：香港保健协会资助项目!第 96 0 0 86号

摘　　要：目的 :探讨消瘀片减少粥样硬化血管壁中平滑肌细胞数的作用机理。方法 :雄性新西兰兔高脂饮食加腹主动脉内膜剥脱术制成腹主动脉粥样硬化模型 ,通过放免法、比色法、原位末端标记及图像分析技术分别测定连续给予消瘀片 0 .1 6～ 0 .32g/(kg·d)治疗 1 6周后血管壁组织中ET、NO及细胞凋亡的变化。结果 :服用消瘀片后的兔粥样硬化血管壁组织中ET含量降低 ,NO含量增加 ,凋亡细胞阳性反应颗粒所占的面积和积分光密度值减少。结论 :消瘀片通过降低血管壁中的ET而抑制平滑肌细胞的增生 。OBJECTIVE:To investigate the action mechanism of Xiaoyu tablets on reduction of smooth muscle cells(SMC) in atherosclerotic vessel wall.METHOD:The atherosclerotic model was generated with male New Zealand rabbits that were given high fat diet and abrasion of the abdominal aorta endothelial cells.The rabbits were then administered with xiaoyu tablets 0.16～0.32g/(kg·d) for 16 weeks.Changes in endothelin(ET),nitric oxide(NO) and apoptotic cells of atherosclerotic vessel wall were determined by the radioimmunoassay,colorimetric method and the techniques of DNA in situ end labeling and image pattern analysis,respectively.RESULTS:The ET in atherosclerotic vessel wall was decreased,NO was increased and the area and integral optical density of labeled apoptotic nuclei were markedly decreased.CONCLUSION:Xiaoyu tablets not only inhibited proliferation of SMC through reducing ET in atherosclerotic vessel wall,but also induced apoptosis of proliferated SMC and enhanced the recognition and phagocytosis of phagocyte or SMC on apoptotic cells by means of increasing NO in vessel wall.

关 键 词：消瘀片 动脉粥样硬化 内皮素 一氧化氮 凋亡 

分 类 号：R285.5[医药卫生—中药学]