氧化应激对人胰腺导管上皮细胞增殖的影响  

Effect of oxidative stress on the proliferation of human pancreatic ductal epithelial cells

作　　者：戴欣欣[1] 郝建宇[2] 

机构地区：[1]首都医科大学附属北京朝阳医院超声医学科,100020 [2]首都医科大学附属北京朝阳医院消化科,100020

出　　处：《北京医学》2014年第1期1-4,F0003,共5页Beijing Medical Journal

基　　金：北京市教育委员会科技发展计划面上项目(KM201010025028)

摘　　要：目的探讨氧化应激对人胰腺导管上皮细胞增殖的影响。方法不同浓度过氧化氢(H2O2)作用于人胰腺导管上皮细胞6～48 h,采用四甲基偶氮唑盐(MTT)比色法检测细胞光密度(OD),绘制细胞生长曲线,计算细胞存活率;采用碘化丙啶(PI)单染色法检测细胞周期,计算细胞增殖指数(proliferation index,PI’)。结果细胞处理6 h,H2O2300μmol/L组存活率下降[(79.10±9.21)%vs.(100.00±14.84)%,P<0.05]。12 h H2O2300μmol/L组OD值降低(0.277±0.022 vs.0.309±0.015;P<0.05),存活率明显下降[(56.90±29.91)%vs.(100.00±20.91)%,P<0.01]。24h H2O2100、200、300μmol/L组OD值明显降低(0.493±0.011,0.434±0.029,0.373±0.014 vs.0.529±0.011;P<0.01),存活率明显下降[(87.95±3.73)%,(67.61±9.87)%,(46.84±4.76)%vs.(100.00±3.85)%;P<0.01],PI’明显降低[(25.50±1.81)%,(25.20±1.80)%,(21.11±1.78)%vs.(32.20±2.33)%;P<0.01]。48 h H2O225μmol/L组OD值明显升高(0.683±0.014 vs.0.587±0.024,P<0.01),存活率明显升高[(127.39±3.99)%vs.(100.00±6.72)%;P<0.01],H2O225、50μmol/L组PI’明显升高[(44.20±4.75)%,(40.50±2.67)%vs.(35.40±1.25)%;P<0.01];而H2O2100、200、300μmol/L组OD值明显降低(0.533±0.011,0.440±0.018,0.376±0.003 vs.0.587±0.024;P<0.01),存活率明显下降[(84.74±3.08)%,(58.20±5.10)%,(39.87±0.71)%vs.(100.00±6.72)%;P<0.01],PI’明显降低[(24.30±1.83)%,(21.97±1.82)%,(16.94±3.55)%vs.(35.40±1.25)%;P<0.01]。结论低浓度H2O2显著促进人胰腺导管上皮细胞增殖,呈时效及量效关系;反之,高浓度H2O2抑制细胞增殖。Objective This study was undertaken to investigate the effect of oxidative stress on the proliferation of cuhured human pancreatic ductal epithehal cells. Methods Human pancreatic ductal epithelial ceils were exposed to different concentrations of hydrogen peroxide （H202） for 6 and 48 hours. Cell viability and growth rate were estimated using methyl thiazolyl tetrazolium （M33＂）. Propidium iodide （PI） was used for cell cycle measurement. Proliferation index （PI＇） was calculated using traditional methods. Results After cells were treated for 6h, cell livability was decreased in the H202 300 Ixmol/L group [（79.1＋9.21）% vs. （100.00＋14.84）%; P 〈 0.05]. At 12h, the OD-value was decreased [（0.277＋ 0.022） vs. （0.309~0.015）; P 〈 0.05] and cell livability was obviously decreased [（56.90~29.91）% vs. （100.00~20.91）%; P 〈 0.01] in the 3001~mol/L H202 group. At 24 h, the OD-values were all markedly decreased in the 100, 200, 300txmol/L H202 group （0.493＋0.011, 0.434~0.029, 0.373＋0.014 vs. 0.529~0.011; P 〈 0.01）, cell livability was all conspicuously decreased [（87.95~3.73）%, （67.61~9.87）%, （46.84~4.76）% vs. （100.00~3.85）%; P 〈 0.01], PI＂ was also conspicuously decreased [（25.50~1.81）%, （25.20~1.80）%, （21.11＋1.78）% vs. （32.20~2.33）%; P 〈 0.01]. At 48 h, OI）,value was conspicuously increased （0.683~0.014 vs. 0.587~0.024; P 〈 0.01） and cell livability was also conspicuously increased [（127.39~3.99）% vs. （100.00~6.72）%; P 〈 0.01] in 25 Ixmol/L H202 group, PI＂ was both conspicuously increased in the 25, 50 vmol/L H202 group [（44.20＋1.75）%, （40.50~1.82）% vs. （35.40~1.82）%; P 〈 0.01]. Whereas, the OD-values were all conspicuously decreased in 100, 200, 300 p^mol/L H202 group （0.533~0.011, 0.440~0.018, 0.376~0.003 vs. 0.587~0.024; P 〈 0.01）, cell inability was conspicuously decreased [（84.74＋3.08）%, （58.20~5.10）%, （39.87~0.71）% vs. （100.00~6.72）%; P 〈 0.01], PI＂ was a

关 键 词：氧化应激 人胰腺导管上皮细胞 增殖 过氧化氢 

分 类 号：R587[医药卫生—内分泌]