新制抗癌方对实验性肝癌抑癌基因的影响  

Effects of Newly-made Anti-cancer Recipe on Cancer-restrained Gene of Experimental Liver-cancer

作　　者：郭振球[1] 朱梅菊[2] 郭绶衡 周小舟 陈新宇[5] 肖长江[5] 贺运河[5] 

机构地区：[1]湖南中医学院中医诊断研究所,湖南长沙410007 [2]湛江师范学院,广东湛江524000 [3]湖南省卫生防疫站,湖南长沙410000 [4]深圳中医院,广东深圳518000 [5]湖南中医学院第一附属医院,湖南长沙410007

出　　处：《湖南中医学院学报》2000年第4期1-5,共5页Journal of Hunan College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(39770917)

摘　　要：为研究抗癌方对抑癌基因的影响 ,并证实其抗癌疗效。采用人肝癌细胞HepG2 培养 ,人肝癌细胞裸鼠移植及血清药理学方法 ,分析抗癌方对人肝癌细胞株HepG2 的影响 ,用LSAB法分析P5 3 ,免疫组化法分析HepG2 P2 1waf1/Cip1蛋白表达 ;并对人肝癌细胞裸鼠复种移植瘤 ,用光镜、电镜观测其细胞形态学变化和P2 1蛋白基因表达。结果显示 ,抗癌方能明显抑制HepG2 的生长 ,对HepG2 细胞的半数生长的抑制剂量 (IC 5 0 )为 1mg/ml。LSAB方法观察发现抗癌方能诱导HepG2 细胞株从相当弱到非常强的比较致密的细胞核染色 ,且低浓度(0 .1mg/ml)P5 3蛋白表达诱导作用强于高浓度 (1mg/ml) ,将近 5 0 %的细胞出现P5 3高表达 ,抗癌方血清各组人肝癌细胞HepG2 P2 1waf1/Cip1表达高于CTX组及空白血清组 (均P <0 .0 1)。大剂量组HepG2 P2 1waf1/Cip1表达高于中剂量组及小剂量组 (均P <0 .0 5 )。CTX血清组和空白血清组HepG2 P2 1waf 1/Cip 1表达无显著性差异(P >0 .0 5 )。对人肝癌细胞裸鼠复种移植瘤治疗后抗癌方组和CTX组移植瘤体和重量分别小于空模组 (均P <0 0 1) ,抑癌率为 33 %。抗癌方组癌组织HepG2 P2 1蛋白表达增强 ,而空模组、CTX组HepG2 P2 1蛋白表达均极低。电镜观察 :抗癌方组癌细胞完整 ,核染色质浓缩并边集 。Aim:To study the effects of newly-made made anti-cancer recipe Kangqianling in Prorenting and treating liver fibrosis.Method:The experiment adopted human liver-cancer's cell HepG 2 cultivation,human liver-cancer cell rat-naked replanted carcinoma and methods of serum pharmaco-logy,and analysed effect of anti-cancer recipe on human liver-cancer's cell plant HepG 2 cells,and analysed P53 with LSAB method,expression of HepG 2 cells P21waf1/Cip1 protein with immunity-histochemistry method and observed its varieties of cellular morphology and expression of P21 protein gene with optical microscope and electron microscope in human liver cancer-cell rat-naked replanted carcinoma. Results:Anti-cancer recipe could restrain HepG 2 growth in evidence and its half growth restraint dose (IC 50)was 1 mg/ml.With LSAB method we observed that anti-cancer recipe was able to induce from very feeble to very strong and thick karyon pigmentation in HepG 2 cell plants and its low concentration(0.1 mg/ml)was stronger than its high one(1 mg/ml)in P53 protein expression and nearly 50% of cell show P53 high expression.HepG 2 P21waf1/Cip1 of human liver's cancer cells in serums of all groups of anti-cancer recipe had higher expression than those of CTX group and blank serum group(all P<0.05).HepG 2 P21waf1/Cip1of big dose group of anti-cancer recipe was higher expression than one of its middle and small dose groups(all P<0.05).HepG 2 P21waf1/Cip1 of both CTX serum and blank serum had no significant difference of its expression(all P>0.05).After human liver cancer-cell rat-naked replanted carcinoma were treated,replanted bodies'sum and weight of both anti-cancer recipe and CTX groups were respectively smaller than that of blank model group(all P<0.01),and their rate of restraining-cancer was 33%.and in the group of the anti-cancer recipe expression of HepG 2 and P21 of cancer tissue became strong and in the groups of bland model and CTX their expression was terribly low.With electron microscope we observed:in the group of anti-c

关 键 词：抗癌方 实验性肝癌 HepG_(2) P21蛋白 P53蛋白 细胞凋亡 

分 类 号：R273.57[医药卫生—中西医结合]