高血糖条件下神经降压素调节巨噬细胞炎症反应的实验研究  

The Experimental Study of Neurotensin Modulating the Inflammatory Response of Macrophages under Hyperglycemic Conditions

作　　者：张慧宇[1] 郭敏芳[2] 刘华[2] 张艳霞[2] 

机构地区：[1]山西大同大学医学院中医系,山西大同037009 [2]山西大同大学医学院组胚教研室,山西大同037009

出　　处：《山西大同大学学报（自然科学版）》2013年第6期42-45,共4页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：山西大同大学2011年度青年科学研究基金项目[2011Q13]

摘　　要：目的观察神经降压素在高血糖条件下对巨噬细胞的影响。方法取昆明小鼠脾巨噬细胞,高糖培养液培养15 d,细胞分PBS对照组和神经降压素干预组。ELISA检测法检测TNF-、IL-1β及IL-6的释放;RT-PCR检测TNF-、IL-1β及IL-6 mRNA的表达。结果高糖条件下神经降压素能降低巨噬细胞TNF-、IL-1β及IL-6的释放和mRNA的表达(P<0.05)。结论在高糖条件下,神经降压素可以通过抑制炎性细胞因子的释放影响巨噬细胞的反应。Objective To observe the effect of Neurotensin （NT） in macrophages function under hyperglycemic conditions. Methods Macrophages were prepared from spleen of Kunming mice and incubated in 30mM （high glucose） D-glucose, for 15 days. The ceils were divided into the PBS control group and Neurotensin treatment group. The level of TNF-α, IL-1β and IL-6 was detected by ELISA; The mRNA expressions of TNF-α, IL-1β and IL-6 were detected using RT-PCR. Results： NT can decrease the release of TNF-α, IL-1β and IL-6 in macrophages under hyperglycemic conditions （P〈0.05）. Conclusion These studies demonstrate that NT through the regulation of inflammatory cytokine expression affects macrophage responses.

关 键 词：高血糖 神经降压素 巨噬细胞炎症反应 

分 类 号：R587.1[医药卫生—内分泌] R392.1[医药卫生—内科学]