单细胞凝胶电泳法检测苯作业工人淋巴细胞DNA损伤  

Study of DNA damage among workers exposed to benzene by alkaline single cell gel electrophoresis detection

作　　者：邢彩虹[1] 李桂兰[1] 李玉英[2] 曲青山 常平[1] 穆瑞东[2] 王援相[2] 尹松年[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050 [2]天津市劳动卫生职业病研究所 [3]纽约大学医学中心环境医学研究所

出　　处：《中华劳动卫生职业病杂志》2000年第5期257-259,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　用碱性单细胞凝胶电泳技术检测苯作业工人外周血淋巴细胞DNA损伤 ,研究苯的遗传毒性。方法　采用碱性单细胞凝胶电泳技术。将接苯工人按累积浓度、历史平均浓度和测定当时 8h时间加权平均浓度 (8hTWA)分组。观察指标包括DNA断裂分级 (将DNA断裂损伤细胞按其损伤程度分级 )及DNA彗星尾长 (彗头末端到彗尾的长度 )。结果　接苯工人DNA断裂程度及DNA彗星尾长 [lg(尾长 +1) ]两个参数 ,接苯组按累积浓度、历史平均浓度和 8hTWA浓度计算 ,均明显高于对照组 ,差异有显著性 (P<0 .0 1) ,并有明显的剂量 -反应关系。结论　彗星试验能够快速、敏感地检测苯引起的人类淋巴细胞DNA损伤 ,提示对于检测环境致癌物和致突变物可能是一有用工具。Objective To detect DNA damage in peripheral lymphocytes of workers exposed to benzene by alkaline single cell gel electrophoresis and to study the genetic toxicity of benzene. Methods Alkaline single cell gel electrophoresis(Comet assay) was used.The workers were divided into different groups by levels of accumulative exposure,historical average and the eight-hour time weighted average(8 h TWA).The grade of DNA breakage and tail length of Comet assay were used to measure DNA damage. Results The grade of DNA breakage,the tail length[lg(tail+1)] and levels of accumulative exposure,historical average and 8 h TWA were significantly higher in lymphocytes of exposed workers,compared to matched controls( P <0.01).The dose-response relationship was significant in the above three benzene concentrations. Conclusion Comet assay could sensitively and more conveniently detect DNA damage induced by benzene in human lymphocytes.It may be a useful tool for human biomonitoring,particularly for the analysis of environmental mutagens and carcinogens.

关 键 词：彗星试验 DNA损伤 淋巴细胞 职业性 苯中毒 

分 类 号：R135.12[医药卫生—劳动卫生]