正交试验量效比对法优选卷柏抗SMMC-7721肝肿瘤组分总黄酮的提取工艺  

Optimization of Extraction Process of Total Flavonoids in Selaginellae Herba against Hepatocarcinoma Cells SMMC-7721 by Orthogonal Test Dose-Effect Comparison Method

作　　者：齐冰[1] 包永睿[1] 孟宪生[1] 王帅[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁省组分中药工程技术研究中心,辽宁省现代中药研究工程实验室,辽宁大连116600

出　　处：《中国实验方剂学杂志》2014年第2期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2010ZX09401-304-105C)

摘　　要：目的:优选卷柏抗SMMC-7721肝肿瘤组分总黄酮的提取工艺。方法:选取提取时间、提取次数、乙醇用量及体积分数为考察因素,以人肝癌细胞SMMC-7721抑制率为指标的正交试验结果优选总黄酮和穗花杉双黄酮提取量综合评分的权重系数比,并通过SPSS 17.0软件对有效组分与药效学指标进行Pearson相关系数分析。采用HPLC测定穗花杉双黄酮含量,流动相乙腈-0.5%乙酸水(33∶67),检测波长269 nm。结果:最佳提取工艺条件为加10倍量70%乙醇回流提取1次,每次3 h;穗花杉双黄酮含量与总黄酮含量的权重系数分别为0.7,0.3,反映含量指标与抑制率呈一定相关性。结论:以有效成分含量代表药效作为正交试验指标是科学合理的,优选的提取工艺稳定可行,为中药有效成分的提取提供新思路。Objective： To optimize extraction process of total flavonoids from Selaginellae Herba against hepatocarcinoma cells SMMC-7721. Method： Taking extraction time and times, the amount and concentration of ethanol as factors, result of orthogonal test was adopted to optimize weight coefficient ratio of amentoflavone and total flavonoids extraction amounts in which inhibition rate of human hepatoma SMMC-7721 cells was taken as an indicator, contents of effective components and pharmacodynamic indicators were analyzed with Pearson correlation coefficient by SPSS 17.0 software. The content of amentoflavone was determined by HPLC, mobile phase of acetonitrile-0.5% acetic acid water （33：67）, detection wavelength 269 nm. Result： The best extraction conditions were as follows： reflux extracted 3 h with ten times the amount of 70% ethanol; weight coefficients of contents of amentoflaveone and total flavonoids were 0.7 and 0.3, indicating determination of effective ingredients and inhibition rate had a certain correlation. Conclusion： Taking contents of active ingredients instead of efficacy as the index of orthogonal test was scientific and reasonable; Optimized process was stable and feasible, it could provide new ideas for extraction of active ingredients from traditional Chinese medicine.

关 键 词：卷柏总黄酮 正交试验量效对比 噻唑蓝 药效指标 提取工艺 单因素试验 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]