大孔树脂同时分离纯化淫羊藿中淫羊藿苷和淫羊藿次苷Ⅱ的工艺优选  

Optimization of Separation and Purification Technology for Icariin and Icarisid Ⅱ from Epimedii Folium by Macroporous Adsorption Resin

作　　者：任桂友[1] 刘海萍[1] 王爽[1] 李琼[1] 曾锐[1] 

机构地区：[1]西南民族大学民族医药研究院,成都610041

出　　处：《中国实验方剂学杂志》2014年第2期5-7,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"科技部支撑计划项目(2012BAI27B07);西南民族大学研究生创新型科研项目(CX2014SZ51)

摘　　要：目的:建立大孔树脂同时分离纯化淫羊藿中淫羊藿苷和淫羊藿次苷Ⅱ的工艺条件。方法:以淫羊藿苷和淫羊藿次苷Ⅱ的吸附容量、洗脱率为评价指标,通过静态吸附-洗脱试验筛选大孔树脂型号,采用单因素试验优选淫羊藿苷和淫羊藿次苷Ⅱ的吸附和洗脱条件。结果:选用DM301型大孔吸附树脂,最佳吸附条件为上样液质量浓度7.45 g·L-1,上样液流速3BV·h-1;淫羊藿苷洗脱条件为加45%乙醇4 BV以1.5 BV·h-1的流速进行洗脱;淫羊藿次苷Ⅱ洗脱条件为加60%乙醇5 BV以1.5 BV·h-1的流速进行洗脱;淫羊藿苷、淫羊藿次苷Ⅱ纯度分别达56.23%,67.41%。结论:建立的工艺条件可同时分离纯化淫羊藿苷和淫羊藿次苷Ⅱ,且稳定性好、收率较高、生产成本低,适宜于规模化生产推广。Objective： To optimize separation and purification technology of icariin and icariside Ⅱ from Epimedii Folium by macroporous adsorption resin. Method： Taking adsorption capacity and desorption rate of icariin and icariside Ⅱ as indexes,macroporous resin models were screened by static adsorption-elution test,single factor tests were adopted to investigate adsorption and elution conditions. Result： DM301 macroporous resin was the most suitable one for purification of icariin and icariside Ⅱ,its optimum adsorption conditions were as follows：the concentration of sample solution 7.45 g·L-1,sample flow rate 3 BV·h-1;Elution conditions of icariin was eluting with 4 BV 45% ethanol at 1.5 BV·h-1;Elution conditions of icatiside Ⅱ was eluting with 5 BV 60% ethanol at 1.5 BV·h-1;Under these conditions,purities of icariin and icariside Ⅱ were 56.23% and 67.41%,respectively. Conclusion： These established process conditions could be adopted to separate and purify icariin and icariside Ⅱ at the same time with good stability,high yield and low production cost,which were suitable for industrial production.

关 键 词：大孔树脂 淫羊藿 淫羊藿苷 淫羊藿次苷Ⅱ 分离纯化工艺 静态吸附-洗脱试验 

分 类 号：R284.2[医药卫生—中药学] R283.6[医药卫生—中医学]