肿节风复方含药血清对肝癌HepG2细胞增殖、端粒酶及凋亡的影响  

Effect of Serum Containing Sarcandrae Compound on Proliferation, Telomerase Activity and Cellular Apoptosis of HepG2 Cells

作　　者：朱晓莹[1] 李韬[2] 李盛毅 曾怡[1] 

机构地区：[1]右江民族医学院科学实验中心,广西百色533000 [2]右江民族医学院基础医学院,广西百色533000

出　　处：《中国实验方剂学杂志》2014年第2期109-112,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81071345);右江民族医学院科字[2011]1号

摘　　要：目的:观察肿节风复方含药血清对人肝癌细胞HepG2细胞增殖及凋亡的影响。方法:实验设阴性对照组(NS)、5-氟尿嘧啶(5-FU)组(0.025 g·kg-1)和肿节风复方低、中、高剂量组(2.5,5,10 g·kg-1),灌服于大鼠后,提取含药血清。取上述含药血清分别作用于HepG2细胞24,48,72 h;MTT法测定细胞增殖抑制率;ELISA法测定端粒酶活性;荧光显微法及流式细胞术(Annexin V-FITC/PI法)检测肿节风复方含药血清对HepG2细胞凋亡的影响。结果:与对照组比较,肿节风复方含药血清低、中、高剂量组均能抑制HepG2细胞的增殖(高剂量组抑制率最高达42.5%),呈现与时间-剂量依赖趋势;肿节风中、高剂量组能有效抑制HepG2细胞端粒酶活性,与对照组比较P<0.05;肿节风复方含药血清具有诱导HepG2细胞凋亡的作用,与对照组比较P<0.05,其中中剂量组作用24,48,72 h细胞凋亡率分别为(14.2±0.9)%,(30.6±1.0)%,(61.6±1.5)%。结论:肿节风复方含药血清能抑制HepG2细胞增殖、端粒酶活性,诱导HepG2细胞凋亡。Objective： To investigate effect of serum containing Sarcandrae compound on proliferation,telomerase activity and cellular apoptosis of HepG2 liver cancer cells. Method： Serum containing drugs was prepared by Administration of physiological saline, different concentrations of Sarcandrae compound and 5-fluoro-2,4（1H,3H）pyrimidinedion（5-FU）. Consequently to rats. The serum containing drugswas used totreat HepG2 cells for 24, 48, 72 h. Then the effects of different drugs on proliferation, telomerase and apoptosis of HepG2 cells were observed by MTT, ELISA and Flow cytometry Annexin V-FITC/PI assay, respectively. Result： Compared to control group,proliferation and telomerase activities of HepG2 cells were inhibited with time-dependent manner by serum containing Sarcandrae compound significantly （P〈0.05）. All serum containing Sarcandrae compound could promote the apoptosis of HepG2 cells significantly. For middle dose group at 24,48,72 h the apoptosis rate was （14.2±0.9）%, （30.6±1.0）%, （61.6±1.5）%. Conclusion： Serum containing Sarcandrae compound can inhibit the proliferation and telomerase activity of HepG2 cells, and promote the apoptosis of HepG2 cells.

关 键 词：肿节风复方 血清药理学 肝癌 端粒酶 凋亡 

分 类 号：R285.5[医药卫生—中药学]