夏枯草水提液及水提醇沉上清液降血压药效评价  

Anti-hypertensive Effect of Aqueous Extract and Supernatants of Alcohol Extracting-Water Precipitation of Prunella vulgaris

作　　者：夏伯候[1,2] 刘菊妍 李春[4] 何迎春[1,2] 梁航[1,2] 龚力民[1,2] 彭江丽[1,2] 林丽美[1,2] 

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省中药不良成分快速检测及脱除工程技术研究中心,长沙410208 [3]广州医药集团有限公司,广州510130 [4]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2014年第2期113-116,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09201019);教育部高等学校博士学科点专项科研基金(20124323120004);湖南省自然科学基金项目(13JJ4089);湖南省"十二五"重点学科药学学科项目

摘　　要：目的:探讨夏枯草水提液及水提醇沉上清液对自发性高血压大鼠(spontaneously hypertensive rat,SHR)血压的量效和时效关系。方法:采用水提、水提醇沉2种方法处理夏枯草,以SHR大鼠(n=72)为模型,以卡托普利作为阳性对照,考察不同剂量(2.5,5,10 g·kg-1)的夏枯草水提液和醇沉上清液单次灌胃给药后不同时间点(0,2,5,7,10 h)对SHR大鼠收缩压、舒张压、平均动脉压、心率等指标的影响。结果:给药后2 h夏枯草水提液和醇沉上清液不同剂量均能显著降低SHR收缩压(p<0.05),醇沉上清液高剂量(10 g·kg-1)组能显著降低SHR舒张压(P<0.05);给药后5 h,夏枯草水提液低剂量(2.5 g·kg-1)组开始对SHR收缩压无显著影响,醇沉高剂量(10 g·kg-1)组进一步降低舒张压;给药后7 h,仅醇沉上清液高剂量对SHR收缩压有显著影响(P<0.05),其余各组对SHR舒张压、SHR平均动脉压和心率均无显著性影响。结论:夏枯草水提醇沉所得的上清液在去除部分杂质的情况下保留和富集了夏枯草降血压有效组分,为进一步研究夏枯草降血压物质基础及其制剂工艺提供参考。Objective： To investigatet the time-effect and dose-effect of anti-hypertensive effect in spontaneously hypertensive rats （SHR） byaqueous extract and the supernatants of alcohol extracting-water precipitation of Prunella vulgaris L. Method： P. vulgaris was processed by water extraction, water extraction and alcohol precipitation.The SHR were treated with different dose of two extracts. SBP, diastolic blood pressure（DBP）, mean arterial pressure（MAP）, and heart rate（HR） were measured before and after oral administration for 2,5,7,10 h for determining the time-effect. Result： Compared with the model group, no significant effect were showed on MAP and HR of SHR in all the different dose of test group in every test time points. SBP of SHR decreased significantly in aqueous extract（10, 5 g·kg-1） and the supernatants of alcohol extracting-water precipitation（10, 5, 2.5 g·kg-1） of P. vulgaris,while high dose （10 g·kg-1）of the supernatants of alcohol extracting-water precipitation decreased DBP of SHR after oral administration for 5 h（P〈0.05）. Conclusion： The supernatants of alcohol extracting-water precipitation of P. vulgaris retain and enrich the active anti-hypertension principles when parts of other principles were removed, which can provide reference for the study of anti-hypertension material base and pharmaceutical technology in P.vulgaris.

关 键 词：夏枯草 水提液 水提醇沉 降血压 

分 类 号：R285.5[医药卫生—中药学]