清热解毒方通过下调肠道水通道蛋白治疗脓毒症大鼠机制研究  

Qingre Jiedu Prescription Treating Septic through Down-Regulating AQPs Expression in Rats

作　　者：高巧营[1] 刘大全[1] 吴尚为[1] 李东华[1] 刘洪斌[1] 

机构地区：[1]天津市南开医院中西医结合急腹症研究所,天津300100

出　　处：《中国实验方剂学杂志》2014年第2期133-136,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市科技攻关计划重大项目(05YFGDSF02600);天津市中医药管理局科研项目(11020)

摘　　要：目的:探讨清热解毒方治疗脓毒症模型大鼠的作用机制。方法:采用盲肠结扎穿孔法制作大鼠脓毒症模型。实验分为假手术组、脓毒症模型组、清热解毒方高、中、低3个剂量组(24,12,6 g·kg-1,ig),均1次/d,连续3 d给药。记录各组大鼠死亡情况,并以传统细菌培养法对大鼠标本进行细菌定量检测,通过Western blot法检测大鼠结肠水通道蛋白(AQP)2和AQP8,同时应用血清生化分析仪检测大鼠血清生化指标。结果:模型组病死率56%,且与假手术组大鼠比较,该组大鼠腹水、淋巴结与血液细菌阳性率和细菌含量及血清生化指标明显升高;高剂量组大鼠病死率降低,血清生化指标明显改善,且AQP2和AQP8表达与脓毒症模型组有明显差异性(P<0.05)。各治疗组AQP2和AQP8表达下降,且对中药剂量有依赖性,同时淋巴结与血标本细菌含量显著降低。结论:清热解毒方可通过下调AQPs表达而发挥泻下功能,减少脓毒症大鼠细菌易位治疗脓毒症。Objective： To investigate the mechanism of Qingre Jiedu prescription in treatment septic. Method： The septic model was induced by cecal ligation and puncture. The experimental rats were divided into five groups;control group,septic model group,Qingre Jiedu prescription treated group （24,12,6 g·kg-1）. All animals were treaded via gastrogavage with distilled water and Qingre Jiedu prescription once a day for 3 days. The survival state was observed. The bacterial populations in experimental sepsis were determined by bacterial culture. And aquaporins（AQP） 2 and AQP8 were detected by western blot. Result： The fatality rate in model group was the highest （56%）, meanwhile all bacteria in ascites, lymph nodes and bloods were significantly higher than that in control group. For treaded group, the mortality and the serum biochemical parameter in the high dose group were significantly decreased. The AQP2 and AQP8 expression levels in all treated groups were decreased compared with that in model group, with a dose-dependent manner. In the high dose group,the number of pathogens in lymph nodes and blood was significantly lower than that in model group. Conclusion： Qingre Jiedu prescription can decrease bacterial translocation by reducing expression of AQPs to increase excreta.

关 键 词：清热解毒方 脓毒症 细菌易位 水通道蛋白 

分 类 号：R285.5[医药卫生—中药学]