山楂叶提取物类脂体与益心酮片对大鼠急性心肌缺血药效的比较研究  

Comparative Study of Hawthorn Leaves Extract Niosomes and Yixintong Tablet on Pharmacodynamic Actions of Acute Myocardial Ischemia in Rats

作　　者：鲍慧玮[1] 李婷[1] 孙敬蒙 张炜煜[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国实验方剂学杂志》2014年第2期140-143,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2014]第88号)

摘　　要：目的:对山楂叶提取物类脂质体和市售的益心酮片进行"大鼠急性心肌缺血"药效作用比较,阐明山楂叶提取物类脂质体的药效作用特点。方法:将大鼠随机分为空白对照组、模型组、益心酮片组、山楂叶提取物类脂质体组,每组10只。益心酮片组和山楂叶提取物类脂质体组大鼠均ig给予17.28 mg·kg-1剂量的山楂叶提取物,每天1次,连续7 d;模型组、空白对照组大鼠ig等体积蒸馏水(10 mL·kg-1)。第5天和第6天除空白对照组外,其余各组均在给药后1 h分别ip异丙肾上腺素4.2 mg·kg-1,每天1次,以建立大鼠急性心肌缺血模型;空白对照组大鼠相应ip等量生理盐水。末次给药1 h后,用10%水合氯醛麻醉,腹主动脉取血。以大鼠血清心肌酶[天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、肌酸磷酸激酶(CPK)]活性,丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性及主要脏器系数作为观察指标,比较山楂叶提取物类脂质体及市售益心酮片对"急性心肌缺血"作用特点。结果:与空白对照组比较,模型组AST,LDH,CPK活性和MDA含量均显著升高(P<0.05),SOD活性极显著性降低(P<0.001);与模型组比较,益心酮组仅LDH活性、MDA含量显著性降低(P<0.05),山楂叶提取物类脂质体组AST,LDH,CPK活性均显著性或极显著降低(P<0.05或P<0.01),MDA含量显著性降低(P<0.05),SOD活性极显著性升高(P<0.01);与益心酮组比较,山楂叶提取物类脂体组AST,LDH,CPK活性和MDA含量差异无统计学意义,SOD活性显著性升高(P<0.05)。山楂叶提取物类脂质体对大鼠主要脏器系数未见明显影响。结论:山楂叶提取物类脂体和益心酮片在临床等效剂量下均明显改善急性心肌缺血大鼠的血清心肌酶损伤程度,降低MDA的含量,对异丙肾上腺素所致大鼠心肌缺血损伤具有显著的保护作用,但益心酮片对SOD的活性影响没有显著差异,山楂叶提取物类脂体对SOD的活性显著性升高。Objective： To compare Hawthorn leaves extract niosomes and Yixintong tablet on pharmacodynamic actions of acute myocardial ischemia in rats,and provide the pharmacodynamics characteristics of Hawthorn leaves extract niosomes. Method： The rats were randomly divided into blank control group, model group, Yixintong tablet group, hawthorn leaf extract niosomes group, with 10 rats in each group. The rats of Yixintong tablet group and hawthorn leaf extract niosomes group were administered with 17.28 mg·kg-1 hawthorn leaf extract, once a day, for continuous 7 days;model group and blank control group rats were administered with equal volume of distilled water （10 mL·kg-1）. On the 15th days and 16th day, except blank control group, other groups were ip 4.2 mg·kg-1 1 h after administration of isoproterenol, once a day, in order to establish the rat acute myocardial ischemia model, the rats in blank control group were ip equal volume of physiological saline. 24 h after establishing the model, 1 h after the last administration, the rats were anesthetized with 10% chloral hydrate to obtain blood samples. Some myocardium enzymes like aspartic acid transferase （AST）, lactate dehydrogenase （LDH）, creatine phosphokinase （CPK）, malonyldialdehyde （MDA） and superoxide dismutase （SOD） were measured. Result： Compared with the blank control group, in model group the level of myocardium enzymes and MDA increased significantly （P〈0.05）, the level of SOD was significantly decreased （P〈0.001）. Compared with model group,only the LDH and MDA levels in Yixintong group were significantly decreased（P〈0.05）,AST, LDH, CPK, MDA levels in Hawthorn leaves extract niosomes group were significantly decreased（P〈0.05 or P〈0.01）, the level of SOD was significantly elevated（P〈0.01）. Compared with the Yixintong group, the AST, LDH, CPK, MDA levels in Hawthorn leaves extract niosomes group were no significant difference, the SOD level were significantly elevated（P〈0.05）. Hawthorn leaves

关 键 词：山楂叶提取物类脂质体 益心酮片 药效学实验 

分 类 号：R285.5[医药卫生—中药学]