HPLC法测定胆酸类化合物与牛血清白蛋白的结合率  

Binding Rate of Cholic Acid Compound and Bovine Serum Albumin by HPLC

作　　者：张晓静[1] 李丹丹[1] 张俊义[1] 孙毅坤[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学,北京100102

出　　处：《中国药房》2014年第5期413-415,共3页China Pharmacy

基　　金：国家科技重大专项课题(No.2010ZX09502-002)

摘　　要：目的:研究胆酸类化合物与牛血清白蛋白的结合率,为进一步了解胆酸类化合物体内蛋白结合特性提供依据。方法:采用平衡透析法,以磷酸盐缓冲溶液为透析液,透析内液中加入1 mmol/L牛血清白蛋白1 ml,透析外液中加入质量浓度分别为2.02、1.01、0.51 mg/ml的胆酸和猪脱氧胆酸,振荡30 h后,以高效液相色谱法测定透析袋两侧磷酸盐缓冲溶液中胆酸类化合物的质量浓度,并计算胆酸类化合物与牛血清白蛋白的结合率。结果:3种质量浓度下,胆酸与牛血清白蛋白的结合率分别为78.7%、78.2%、59.4%,猪脱氧胆酸与牛血清白蛋白的结合率分别为68.9%、76.2%、81.8%。结论:胆酸类化合物与牛血清白蛋白具有中等强度的结合率,胆酸类化合物在体内不易因游离浓度过大而产生副作用。OBJECTIVE： To study the binding rate of cholic acid （CA） compounds and bovine serum albumin （BSA）, and to provide reference for the further study of in vivo protein binding characteristics of CA compounds. METHODS： Using phosphate buffer （PBS） as dialysate, 1 mmol/L BSA 1 ml was added into intra-dialysate; and 2.02, 1.01 and 0.51 mg/ml CA and hyodesoxy-cholic acid （HDCA） were added into extra-dialysate in equilibrium dialysis. After oscillated for 30 h, HPLC was employed to determine the concentration of CA compounds in the PBS. The protein binding rates of CA compound and BSA were studied. RESULTS： Under the 3 kinds of concentrations, the binding rates of CA and BSA were 78.7%, 78.2%, 59.4%, respectively; the binding rates of HDCA and BSA were 68.9% , 76.2% and 81.8%, respectively. CONCLUSIONS： There is moderate intensive binding rate between CA compounds and BSA. There is no side effect due to free over-dose of CA compounds in vivo.

关 键 词：胆酸 猪脱氧胆酸 牛血清白蛋白 结合率 高效液相色谱法 平衡透析法 

分 类 号：R969[医药卫生—药理学] Q512.1[医药卫生—药学]