肝细胞癌中HBx的表达及其与患者预后的关系  

The expression of HBx in hepatocellular carcinoma and its relationship with disease-free survival of HCC patients

作　　者：洪清琦[1] 何传超[2] 徐鋆耀[2] 胡斌[3] 王捷[2] 

机构地区：[1]厦门大学附属第一医院肿瘤中心肿瘤外科,福建厦门361003 [2]中山大学孙逸仙纪念医院肝胆外科,广州510120 [3]厦门大学附属第一医院肿瘤中心检验科,福建厦门361003

出　　处：《岭南现代临床外科》2014年第1期26-29,共4页Lingnan Modern Clinics in Surgery

基　　金：国家自然科学基金资助项目(30700803);福建省自然科学基金青年创新项目(2012D-52)

摘　　要：目的检测乙型肝炎病毒x蛋白(HBx)在肝细胞癌中的表达及其与患者预后的关系,并探讨人血清HBx抗原的检测。方法用免疫组化法检测35例肝癌患者肿瘤组织中HBx蛋白的表达,与临床资料进行相关分析;应用酶联免疫吸附法(ELISA)检测77例人血清中HBx抗原的表达情况。结果肝癌组织中HBx的总体阳性检出率为20/35(57.14%),其中乙型肝炎病毒表面抗原(HBsAg)阳性者HBx的阳性率为15/24(62.50%),而HBsAg阴性者HBx的阳性率为5/11(45.45%);HBx阳性与HBx阴性的肿瘤组织低分化比率分别为9/20(45.00%)及2/15(13.33%),差异有统计学意义(P<0.01);HBx阳性肝癌患者的无病生存期(DFS)明显短于HBx阴性患者(Kaplan-Meier法及log-rank检验,P=0.03)。ELISA检测人血清中HBx抗原的总阳性率为48/77(62.34%)。结论肝癌肿瘤组织中HBx的表达与患者无病生存期呈负相关,ELISA法能有效检测人血清中HBx抗原的表达。Objective To detect the expression of HBx in HCC and analyze its relationship with disease-free survival （DFS） in HBV-related HCC. To evaluate the detection performance of HBx antigen in human serum. Methods Immunohistoehemical staining was utilized to detect the expression of HBx in 35 HBV-related HCC tissues, and results were analyzed with clinical data. Enzyme-linked immunosorbent assay （ELISA） was applied to detect the HBx antigen in human serum. Results HBx positive ratios were 20/35 （57.14%） in HCCs, with 15/24 （62.50%） of HBsAg positive and 5/11 （45.45%） of HBsAg negative cases respectively. The poorly differentiated ratio of HBx positive （9/ 20, 45.00%） was significantly higher than that of HBx negative （2/15, 13.33%） cases （P〈0.01）. Disease free survival （DFS） of HBx positive and negative group was estimated by Kaplan-Meier method and compared by log-rank test. The results showed poor DFS in HBx positive group （P=0.03）. ELISA could efficiently detect HBx antigen in human serum with 48/77（62.34%） positive. Conclusions The expression of HBx is correlated with tumor pathological grading and poor DFS of HCC patients. ELISA can efficiently detect HBx antigen in human serum.

关 键 词：肝细胞癌 乙型肝炎病毒 乙型肝炎病毒X蛋白 肿瘤病理分级 无病生存期 

分 类 号：R657.3[医药卫生—外科学]