透皮吸收促进剂对小儿腹泻凝胶膏经皮渗透性的影响  

Effects of Penetration Enhancers on Percutaneous Penetration of Infantile Diarrhea Gel Ointments

作　　者：秦海军[1,2] 马玲[1,2] 张毅[1,2] 陈师农[1,2] 任燕茹[3] 

机构地区：[1]安徽省药物研究所,合肥230022 [2]安徽省中药研究与开发重点实验室,合肥230022 [3]安徽中医药大学药学院,合肥230031

出　　处：《中国实验方剂学杂志》2014年第3期39-41,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:考察不同透皮促进剂对小儿腹泻凝胶膏体外透皮吸收能力的影响。方法:采用药物透皮仪进行小儿腹泻凝胶膏的体外经皮渗透试验,通过单因素试验筛选接受液,并分别考察加入不同质量分数(1%,3%,5%)氮酮、油酸、丙二醇后小儿腹泻凝胶膏剂中吴茱萸碱及吴茱萸次碱的24 h体外累积渗透总量和累积透过率。采用HPLC测定吴茱萸碱及吴茱萸次碱含量,流动相乙腈-水(51∶49),检测波长225 nm。结果:空白样品中吴茱萸(次)碱在24 h内的累积透过率34.53%,含不同质量分数氮酮样品中吴茱萸(次)碱累积透过率分别为33.92%,34.78%,34.65%,含不同质量分数油酸样品则分别为为33.67%,34.57%,34.57%,含不同质量分数丙二醇样品则分别为34.49%,34.61%,34.53%。结论:小儿腹泻凝胶膏剂中不需加入化学透皮吸收促进剂,其所含挥发性成分本身具有促进药物渗透的作用。Objective： To investigate effects of different absorption ability of infantile diarrhea gel ointments. Method： penetration enhancers on in vitro transdermal Drug transdermal instrument was adopted to investigate percutaneous penetration of infantile diarrhea gel ointments, acceptable solution was selected by single factor test, and then 24 h in vitro total cumulative permeation and cumulative transmittance of evodiamine and retaecarpine in infantile diarrhea gel ointments were investigated after adding different content （1% , 3% , 5% ） azone, oleic acid, propylene glycol. HPLC was adopted to determine contents of evodiamine and retaecarpine by taking acetonitrile-water （51： 49） as mobile phase and detection wavelength of 225 nm. Result： Within 24 h, cumulative transmittance of evodiamine and retaecarpine in blank sample was 34.53% , in samples containing different amounts of azone were 33.92%, 34.78% and 34.65%, in samples containing different contents of oleic acid were 33.67% , 34.57% and 34.57% , in samples containing different contents of propylene glycol were 34.49%, 34.61% and 34.53%, respectively. Conclusion： Volatile components from infantile diarrhea gel ointments had promotion role of drug penetration.

关 键 词：小儿腹泻凝胶膏 透皮吸收促进剂 吴茱萸碱 吴茱萸次碱 累积渗透总量 累积透过率 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]