HPLC同时测定五子衍宗丸中5种活性成分的含量  

Simultaneous Determination of Five Ingredients in Wuzi Yanzong Pill by HPLC

作　　者：刘伟[1] 周建良 陈碧莲 祝明 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江省食品药品检验研究院,杭州310004

出　　处：《中国实验方剂学杂志》2014年第3期74-78,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十二五"资助项目(2012ZX09304-005)

摘　　要：目的：建立五子衍宗丸中5种成分金丝桃苷、山柰素、五味子醇甲、五味子甲素和五味子乙素的含量测定方法。方法：AngilentExtendC18色谱柱（4．6mm×250mm，5μm），流动相乙腈-甲醇（10：1）和0．4％磷酸，梯度洗脱柱温30℃，流速1．0mL·min-1检测波长250nm（五味子醇甲、五味子甲素、五味子乙素），360nm（金丝桃苷、山柰素）。结果：在该色谱条件下，金丝桃苷、山柰素、五味子醇甲、五味子甲素和五味子乙素分离良好，分别在4．824～482．4，4．358～87．16，1．854～185．4，5．610—112．2，3．850—77．00ng呈良好的线性关系，加样回收率（n=9）依次为101．2％，98．9％，101．6％，101．3％，97．6％。结论：所建立的方法简便、准确、重复性好，可用于五子衍宗丸的质量控制。Objective： A method for simultaneous determination of five ingredients in Wuzi Yanzong pill was developed by reversed-phase high-performance liquid chromatography （HPLC）. Method： HPLC was performed on Angilent Extend Cls column （4.6 mm x 250 mm, 5 μm） with a gradient elution system using methanol： ethylaetate （ 10：1 ） 4）. 4% phosphoric acid at 30 ℃ and at a flow rate of 1.0 mL .min-1 The eluate was detected by wavelength of 250 nm for schisandrin, deoxyschizandrin, γ-schizandrin and 360 nm for hyperoside and kaempferide. Result： Hyperoside, kaempferide, schisandrin, deoxyschizandrin and γ-schizandrin were baseline separated and their linear ranges were 4. 824-482.4, 4. 358-87.16, 1. 854-185.4, 5. 610-112.2, 3. 850- 77.00 ng, respectively. The recovery of the five compounds （n = 9） were 101.2% , 98.9% , 101.6% , 101.3% , 97.6%, respectively. Conclusion： The developed method is simple, accurate, and shows good precision and reproducibility. It is could be used for the quality control of Wuzi Yanzong pill.

关 键 词：金丝桃苷 山柰素 五味子醇甲 五味子甲素 五味子乙素 高效液相色谱法 五子衍宗丸 

分 类 号：R284.1[医药卫生—中药学]