离体条件下培养物中冬凌草甲素积累与其组织结构关系  

Study on Relationship Between Structure and Accumulation of Oridonin of Cultural Materials in vitro

作　　者：苏秀红[1] 董诚明[1] 史应强[1] 陈随清[1] 冯卫生[1] 张丽萍[1] 刘春朝[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2014年第3期86-90,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173486);河南中医学院创新人才项目(2011XCXRC02);河南省高等学校青年骨干教师计划项目(2011GGJS-089);河南省教育厅科学技术研究重点项目(13A360613)

摘　　要：目的:研究冬凌草甲素动态积累变化关键期培养物显微、超微结构的变化,探讨培养物组织结构与冬凌草甲素积累的相关性。方法:采用高效液相色谱法研究了离体培养过程中培养物内冬凌草甲素积累的动态变化规律。在此基础之上,采用石蜡切片、透射电镜方法研究了培养物组织结构与冬凌草甲素积累的关系。结果:离体培养过程中培养物中冬凌草甲素积累表现出有-无-有的规律性,随着叶片外植体的脱分化,培养物内叶绿体数目逐渐减少,冬凌草甲素的含量也随之逐渐降低,当冬凌草甲素再次积累时,培养物内叶绿体数目再次增多且出现了维管组织的分化。结论:冬凌草甲素积累合成与培养物叶绿体数目的多少及内部维管系统的分化有着紧密的联系。Objective： Structure and uhrastructure of cultural materials were studied to explore the correlation between the structure and the accumulation of oridonin. Method： High performance liquid chromatography was used to determine the changes of oridonin in vitro Rabdosia rubescens. On the basis of it, the anatomy of cuhural materials of R. rubescens were compared using light microscopy, and transmission electron microscopy. Result： The content of oridonin was higher in donor plant （leaf） tissues, but as they began to dedifferentiation into callus, oridonin were absent in the dedifferentiation callus, when the calli initiated organogenesis （bud primordial）, it started appearing. As the leaf dedifferentiated into callus the number of chloroplast decreased. At the same time, the content of oridonin was decline until absent. But when the oridonin accumulated, the number of chloroplast increased again and the differentiation of vascular was founded in the cultural materials. Conclusion： The result showed that the number of chloroplast and differentiation of vascular played key role in biosynthetic pathway of oridonin.

关 键 词：冬凌草 冬凌草甲素 愈伤组织 叶绿体 

分 类 号：R282[医药卫生—中药学]