基于生理病理条件考察藜芦对人参抗疲劳作用的影响  

Influence of Veratrum nigrum to Panax gingseng's Anti-fatigue Based on Uniform Design

作　　者：林雅[1,2] 尚尔鑫[3] 徐颖[2] 杨悦[2] 宋凤瑞[4] 刘志强[4] 林娜[1,2] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]中国中医科学院中药研究所,北京100700 [3]南京中医药大学药学院,南京210046 [4]中国科学院长春应用化学研究所、长春质谱中心,长春130022

出　　处：《中国实验方剂学杂志》2014年第3期124-128,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)(2011CB505300,2011CB505305)

摘　　要：目的：通过均匀设计法考察不同生理病理条件下藜芦对人参抗疲劳作用的影响。方法：ICR小鼠按体重随机分为正常组及气虚组，通过限制饮食加大黄泻下法制备气虚模型。采用均匀设计法，按2因素7／11水平分组给药，观察不同配比及剂量的人参与藜芦合煎液对正常及气虚小鼠低温游泳时间的影响。结果：对正常小鼠的影响：人参对正常小鼠抗疲劳作用的剂量范围为1．00～8．00g·kg-1。当人参：藜芦〈100：1、人参＋藜芦的总剂量在1．00—9．00g·kg-1范围内时，藜芦拮抗人参的抗疲劳作用随剂量及配比的增加增强，但藜芦仅降低人参的效价而不影响其效能。对气虚小鼠的影响：人参对气虚小鼠抗疲劳作用的剂量范围为0．50～8．00g·kg-1。当人参＋藜芦的总剂量在0～90g·kg-1范围内，小鼠游泳时间仅与人参的剂量呈正相关，未观察到藜芦对人参抗疲劳作用的影响。结论：针对不同生理病理条件下的小鼠，人参抗疲劳作用所需剂量略有不同，藜芦对人参抗疲劳作用的影响也不同。Objective： To evaluate the influence of Veratrum nigrum L. to Panax gingseng＇s anti-fatigue effect with the various physiological and pathological conditions. Method： ICR mice were device two groups, the normal and Qi deficiency groups. The Qi deficiency mice were constructed by administrating of Rhei Radix et Rhizoma and on diet. The uniform design method was used by two factors and seven/eleven levels to investigate the hypothermal swim time in the normal and Qi deficiency mice. Result： For normal mice, dose range of P. gingseng＇s anti-fatigue effect was 1.00-8.00 g ·kg-1. When P. gingseng： V. nigrum 〈 100： 1, the total dose was 1.00-9.00 g · kg-1, V. nigrum had the antagonism effect to P. gingseng and the influence would enhance with the increasing of dose. But V. nigrum only decreased the potency not the efficacy of P. gingseng. For Qi deficiency mice, dose range of P. gingseng＇s anti-fatigue effectwas 0.50-8.00 g ·kg-1. WhenP. gingseng： V. Nigrum 〈 100： 1, the total dose was 0-9.00 g ·kg-1, there was a positive correlation between the hypothermal swim time and the dose of P. gingseng. V. nigrum could not affect the anti-fatigue function of P. gingseng. Conclusion ： The mice with the various physiological and pathological conditions, dose of P. gingseng＇s anti-fatigue effect were different, and the influence of V. nigrum to P. gingseng＇s anti-fatigue effect was different either.

关 键 词：十八反配伍 人参 藜芦 抗疲劳 

分 类 号：R285.5[医药卫生—中药学]