菝葜乙酸乙酯提取物对THP-1源性荷脂细胞胆固醇蓄积及SREBP-1表达的影响  

Effect of Ethyl Acetate Extract from Smilax china on Cholesterol Accumulation and SREBP-1 Expression in THP-1-derived Lipid-loaded Cells

作　　者：匡双玉[1,2] 李熠[3] 匡稳定[2] 刘塔斯[1] 

机构地区：[1]湖南中医药大学药学院,长沙410007 [2]南华大学附属第二医院,湖南衡阳421001 [3]南华大学医学院,湖南衡阳421001

出　　处：《中国实验方剂学杂志》2014年第3期137-140,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省研究生科研创新项目(CX2012B344);长沙市科技局计划项目(K1203018-31)

摘　　要：目的:观察菝葜乙酸乙酯提取物对人单核/巨噬细胞系(THP-1)源性荷脂细胞胆固醇蓄积的影响,并初步探讨类固醇调节因子结合蛋白-1(SREBP-1)蛋白表达在其中的作用。方法:以THP-1源性荷脂细胞为模型,洛伐他汀(80 mg·L-1)做阳性对照,菝葜乙酸乙酯提取物不同浓度(0,40,80,160 mg·L-1)作用于细胞24 h以及80 mg·L-1作用于细胞不同时间(0,12,24,48 h),高效液相色谱法测定细胞内总胆固醇(TC),游离胆固醇(FC),胆固醇酯(CE)的含量,油红O染色观察菝葜乙酸乙酯提取物对THP-1源性荷脂细胞中脂滴形成的影响,Western blot印迹法检测细胞内SREBP-1蛋白表达变化。结果:菝葜乙酸乙酯提取物明显降低THP-1源性荷脂细胞中脂滴含量,细胞内TC,FC,CE水平呈现一定的剂量和时间依赖性下降趋势,同时SREBP-1蛋白表达上调。结论:菝葜乙酸乙酯提取物能引起THP-1源性荷脂细胞TC,FC,CE含量下降,且这种作用与SREBP-1蛋白表达上调有一定关系。Objective： To observe the effect of ethyl acetate extract from Smilax china on the cholesterol metabolism in monocyte/macrophage cell line （THP-1） -derived lipid-loaded cells, and to investigate the effect of the expression of sterol regulatory element binding protein （SREBP-1） and caveolin-1 in this process. Method： The ethyl acetate extract with different density （0,40,80,160 mg ·L-1） were used to detect the level of total cholesterol （TC）, free cholesterol （FC）, cholesterol ester （CE） by using high performance liquid phase analytic process and the expression of SREBP-1 by Western blot at different time （0,12,24,48 h） in THP-1 derived lipid- loaded cells. Result： The ethyl acetate extract from S. china had some effects on THP-l-derived lipid-loaded cells, the lipid droplet density decreased in cells and the level of TC, FC, CE was decreased with a dose and time- dependent manner. The expression of SREBP-1 was upregulated. Conclusion： The ethyl acetate extract from S.china can decreased the level of TC, FC, CE in THP-1 derived lipid-loaded cells, and it is contributed to the up- regulation of SREBP-1 expression at some degree.

关 键 词：菝葜 乙酸乙酯提取物 胆固醇 类固醇调节因子结合蛋白-1 

分 类 号：R285.5[医药卫生—中药学]