肠炎清对溃疡性结肠炎模型大鼠中分泌型免疫球蛋白A和P选择素的影响  

Effects of Changyanqing Decoction on the Expressions of sIgA and P-selection in Ulcerative Colitis Rats

作　　者：傅永锦[1] 张小牧[1] 连大卫 吕俊华[2] 梁基智[1] 潘竞锵[1] 

机构地区：[1]广州市中医医院,广州510130 [2]暨南大学药学院,广州510632

出　　处：《中国实验方剂学杂志》2014年第3期148-152,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省中医药局科研基金项目(2009311);广州市中医药中西医结合科研项目(2009A020)

摘　　要：目的:探讨肠炎清对免疫复合法诱致溃疡性结肠炎模型大鼠结肠组织中分泌型免疫球蛋白A(sIgA)和P选择素的影响研究。方法:SD大鼠64只,雌雄各半,随机分为正常对照组(12只)和模型组(52只);模型组应用免疫复合法(注射抗原乳化剂+TNBS/乙醇)建立溃疡性结肠炎大鼠模型,正常组注射生理盐水。再喂养3周后,随机从造模组中抽取4只处死,检查结肠病变情况,确定溃疡性结肠炎模型建立;随后将造模大鼠随机分为4组:肠炎清低、高剂量组(10,40 g·kg-1),柳氮磺吡啶(SASP,0.1 g·kg-1)组和模型对照组;每组12只,每天灌胃给药1次,连续给药4周。每周观察大鼠疾病活动指数(DAI);末次给药后,处死动物,解剖观察并评分结肠黏膜损伤指数(CMDI);光镜下观察结肠组织的病理学变化,并做组织病理变化评分(HS);采用ELISA方法测定结肠组织中sIgA的含量变化;采用免疫组化法(SP法)检测P选择素的表达变化。结果:与正常组比较,模型大鼠的DAI,CMDI,结肠HS评分和髓过氧化物酶(MPO)均明显上升(P<0.01);结肠组织中的sIgA含量显著下降,而P选择素表达明显增加(P<0.01);与模型组比较,肠炎清治疗4周后大鼠的DAI,CMDI,结肠HS评分和MPO值均能显著下降(P<0.01);结肠组织中sIgA的浓度升高,同时减少了P选择素表达(P<0.01)。结论:肠炎清对免疫复合法诱致溃疡性结肠炎模型大鼠具有治疗作用,其机制可能与提升结肠组织内sIgA浓度和降低P选择素的表达有关。Objective： To investigate the effects of Changyanqing decoction on the expressions of sIgA and P-selection on ulcerative colitis rats induced by immune-combined TNBS/ethanol. Method： Sixty-four healthy rats were divided into 2 groups. Normal control group was injected saline and the model group was established with immune-combined TNBS/ethanol. The UC model rats were randomly divided into model group, Changyanqing group （40 g ·kg 1）, Changyanqing group （10 g ·kg-1） and SASP group （0. 1 g ·kg-1）. All model rats were treated for 4 weeks. During the 4-week treatment, disease activity index （DAI） were observed. At the end of treatment, morphological observation was carried out on the rats＇ colon [ colonic mucosal damage index （CMDI） scores and histological score （HS）]. The activity of myeloperoxidase （MPO） was measured by biochemical method; sIgA was determined by ELISA; P-selection was determined by immunohistochemical staining （SP method）. Result： DAI, CMDI, HS, MPO in model rats were obviously increased （P 〈0.01 ） ; P-selection was also markedly higher in colon （P 〈 0.01 ） ; whereas sIgA was significantly decreased in UC rats compared to normal rats （P 〈0.01）. Compared with the UC model group, Changyanqing （10, 40 g ·kg-1） DAI, CMDI, HS, MPO were obviously reduced （P 〈 0.01 ） ; Compared with the UC model group, Changyanqing （10, 40 g· kg-1） sIgA was significantly increased in the rats （P 〈0.01 ）, whereas P-selection was markedly reduced in the colon （P 〈 0.01 ）. Conclusion： Therapeutic effect of Changyanqing on ulcerative colitis rats is obvious, its mechanisms may be related to deceasing P-selection and enhancing sIgA.

关 键 词：溃疡性结肠炎 肠炎清 分泌型免疫球蛋白A P选择素 

分 类 号：R285.5[医药卫生—中药学]