丹参饮加味方诱导胃癌SGC-7901细胞凋亡及其机制研究  

Experimental Study on Effect and Mechanism of Jiawei Danshen Decotion on Apoptosis of Gastric Cancer SGC-7901 Cells

作　　者：蒋时红[1] 张珊珊[1] 刘燕[1] 

机构地区：[1]河南中医学院方剂学科,郑州450046

出　　处：《中国实验方剂学杂志》2014年第3期166-169,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关项目(122102310189)

摘　　要：目的:探讨丹参饮加味方体外诱导胃癌SGC-7901细胞凋亡的分子机制。方法:以丹参饮加味方120,240,480 mg·L-1作用细胞24,48,72,96 h后,应用四甲基偶氮唑盐法(MTT)和流式细胞仪检测该方对胃癌SGC-7901细胞的抑制率和凋亡率的影响;采用免疫组化法检测丹参饮加味方对Bcl-2,Bax蛋白表达的影响。结果:与空白对照组相比,丹参饮加味方各组细胞抑制率、凋亡率明显升高(P<0.05),Bax蛋白表达量显著增强(P<0.05),Bcl-2蛋白表达量明显降低(P<0.05)。结论:丹参饮加味方能体外抑制胃癌细胞的生长,其诱导SGC-7901细胞凋亡的作用机制可能与上调Bax,下调Bcl-2蛋白的表达有关。Objective： To observe the apoptosis molecular mechanism of gastric cancer SGC-7901 cells induced by Jiawei Danshen decoction. Method： After gastric cancer SGC-7901 cells treated with Jiawei Danshen decotion for 24, 48, 72 and 96 h, cell growth inhibition rate and apoptosis rate were detected by MTT and flow cytometry. The immunocytochemistry method was used to study the effects of Jiawei Danshen decoction on the expression of Bcl-2 and Bax in SGC-7901 cells. Result： the inhibition rate of SGC-7901 induced by Jiawei Danshen decoction was higher than that of negative control group （P 〈 0.05）. The result of Apoptosis rate showed SGC-7901 cells induced by Jiawei Danshen decoction has a higher apoptosis rate compared with the negative control group （P 〈 0.05）. After treated with Jiawei Danshen decoction, the expression of Bcl-2 was obviously decreased and the expression of Bax was obviously increased. Conclusion： The Jiawei Danshen decoction can induce apoptosis of SGC-7901 cells. The mechanism may be relate to its regulation on the expression of Bcl-2 and Bax.

关 键 词：丹参饮加味方 胃癌细胞株 细胞凋亡 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]