黔岭淫羊藿总黄酮对抗维甲酸致大鼠骨质疏松作用的研究  

Resistance of Total Flavones of Epimedium leptorrhizum Stearn on rat Osteoporosis Induced by Retinoic Acid

作　　者：回连强[1] 曹春雨[1] 刘婷[1] 郝然[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2014年第3期170-174,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项课题重大新药创制课题(2009ZX09103-426)

摘　　要：目的:观察黔岭淫羊藿总黄酮(YYH-C)对抗维甲酸致骨质疏松大鼠的作用。方法:将大鼠按体重随机分为对照组、模型组、仙灵骨葆胶囊(0.3 g·kg-1)、YYH-C低、中、高剂量(生药0.7,1.4,2.8 g·kg-1)组共6组,除对照组外,其余各组动物每日灌胃给予维甲酸70 mg·kg-1,连续14 d。造模同时,各组给予相应药物进行干预,共28 d。取左侧股骨和腰椎骨,进行骨计量学指标测定,包括骨密度、骨钙、磷含量和骨灰重/干重百分比,取右侧股骨进行骨组织形态学观察。结果:给药28 d后,与对照组相比,模型组大鼠椎骨的骨密度和股骨的骨灰重/干重比明显下降(P<0.01,P<0.05),股骨、椎骨的骨组织钙和磷的含量均显著降低(P<0.01),形态学检查可见股骨骨皮质变薄,骨髓腔扩大,骨小梁变薄,排列稀疏、不规则,成骨细胞数明显减少等骨质疏松表现。与模型组比较,YYH-C高剂量组能显著增加骨质疏松大鼠腰椎骨的骨密度以及灰重/干重比(P<0.01,P<0.05),YYH-C各剂量组均能不同程度增大股骨灰重/干重比(P<0.01,P<0.05),增加股骨、腰椎骨的钙、磷的含量(P<0.01,P<0.05);显著提高骨皮质面积百分比、扩大骨小梁面积(P<0.01,P<0.05);YYH-C中、高剂量组可使骨质疏松大鼠骨小梁的厚度和面积百分比显著增加,成骨细胞数增多(P<0.01),而中剂量组还能明显减少破骨细胞数(P<0.05)。结论:YYH-C能有效防止维甲酸造成的大鼠骨量丢失,改善骨组织显微结构,调节骨生成与骨破坏的紊乱状态,从而有效对抗骨质疏松的发生。Objective： To observe the effects of total flavones of Epimedium leptorrhizum Stearn （YYH- C） on rat osteoporosis induced by retinoic acid. Method： Rats were divided into control group, model group, Xianling Gubao capsule group （0.3 g ·kg-1 ） and YYH-C lower, middle, and high dose （ raw materials 0.7, 1.4, 2.8 g ·kg-1） groups. Except the control group, eachgroups were administrated retinoic acid 70 mg ·kg-1 in the first 14 consecutive days. At the same time, the different treatment groups were given the corresponding drug to intervene for 28 days. The vertebrae bone and femur bone of left side were dissected to examine the parameters of bone, including bone mineral density （BMD） , bone Ca, bone P, and ash weight/dry weight percentage; the femur bone of right side were dissected to assess the morphology of bone. Result： After 28 days, the BMD of vertebrae bone and ash weight/dry weight percentage of femur bone of the model rats were obviously lower, compared with control group （P 〈0.01, P 〈0.05）. Ca and P content of bone of the model rats were also significantly lower than those of control group （P 〈 0.01 ）. Morphological examination showed osteoporosisperformance such as femoral cortical thinning, bone marrow cavity enlargement, the trabecular bone becaming thin, sparse and irregular, moreover, osteoblast number reduced. YYH-C 2.8 g ·kg-1 group could significantly increase the BMD and ash weight/dry weight percentage of vertebrae bone （P 〈 0.01, P 〈 0.05 ） , meanwhile, three treatment groups of YYH-C could increase the ash weight/dry weight percentage and Ca, P content of the bone, enlarge trabecular area and cortical bone area percentage （P 〈 0.01, P 〈 0.05）. In addition, YYH-C 1.4, 2.8 g · kg-1 groups could increase the thickness and area percentage of trabecular bone （P 〈 0.01 ） , YYH-C of 1.4 g · kg-1 could reduce the number of osteoclasts （P 〈 0.05）. Conclusion： The total flavones of E. leptorrhizum Stearn could resist osteoporosis on r

关 键 词：黔岭淫羊藿总黄酮 维甲酸 骨质疏松 

分 类 号：R285.5[医药卫生—中药学]