白藜芦醇通过下调p38MAPK磷酸化抑制脂多糖诱导的H9c2细胞死亡  

Resveratrol protects H9c2 cells from death induced by lipopolysaccharide via down-regulating the phosphoration of P38MAPK

作　　者：李丽[1] 赵清[1] 李永光[1] 马健[1] 朱伟[1] 魏盟[1] 

机构地区：[1]上海交通大学附属第六人民医院心内科,200233

出　　处：《实用医学杂志》2014年第2期199-201,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金青年基金项目资助(编号:81100099)

摘　　要：目的:研究白藜芦醇是否通过调节p38MAPK活性来抑制脂多糖诱导的H9c2细胞死亡。方法:将H9c2细胞分为对照组(Con)组,脂多糖(LPS)组,白藜芦醇(Res)组,脂多糖+白藜芦醇(RL)组。其中Res组和RL组分别予5μmol/L白藜芦醇预先处理,然后给予LPS组和RL组10μg/mL脂多糖共同孵育30 min和12 h,MTT法检测细胞增殖,流式细胞术检测细胞死亡,Western blot检测p38MAPK磷酸化水平。结果 :LPS使细胞增殖明显减少(P<0.05),死亡数明显增加(P<0.01),p38MAPK磷酸化水平显著升高(P<0.01)。预先给予白藜芦醇处理后,不仅细胞增殖明显增加(P<0.05),细胞死亡数显著降低(P<0.01),而且p38MAPK磷酸化水平明显下降(P<0.01)。结论:白藜芦醇可能通过下调p38MAPK磷酸化抑制脂多糖诱导的H9C2细胞死亡,从而发挥其保护细胞的作用。Objective To investigate whether treating H9c2 cells with Resveratrol attenuates the cell death and apoptosis induced by lipopolysaccharide via regulating the activation of p38 MAPK. Methods H9c2 cells were divided into four groups： control, LPS（10μg/mL lipopolysaecharide） , Res（5μmol/L Resveratrol）, and RL （5μmol/L Resveratrol＋ 10μg/mL LPS）. H9e2 cells from group Res and RL were pretreated with Resveratrol, and then, only cells from group LPS and RL, were incubated with LPS for 30 rain and 12 hours. MTr and Flow cytometry were used to detect cell proliferation and death. The protein level of phosphorylation of p38MAPK was measured by Western blot. Result Compared with control, LPS significantly reduced cell proliferation （P 〈 0.05）, increased （P 〈 0.01）cell death, and up-regulated the level of phosphorylation of p38MAPK （P 〈 0.01）. Pretreatment of Resveratrol attenuated the inhibition of LPS on cell viability and down-regulated the level of phosphorylation of p38MAPK. Conclusion Resveratrol may exert its cytoprotection effects on LPS-induced H9c2 cells via down- regulating the activation of P38MAPK.

关 键 词：白藜芦醇 脂多糖 P38MAPK 

分 类 号：R285[医药卫生—中药学]