复合营养素早期干预对阿尔茨海默病模型小鼠认知功能障碍的影响  

Study on early intervention of compound nutrition for cognitive dysfunction in Alzheimer's disease

作　　者：王超[1] 解微[1] 朱金峰[1] 党瑞[1] 王德才[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150081

出　　处：《卫生研究》2014年第1期103-107,共5页Journal of Hygiene Research

基　　金：国家自然科学基金面上项目(No.81273193)

摘　　要：目的观察复合营养素对阿尔茨海默病模型—APP-PSN双转基因鼠认知功能障碍的早期干预效果。方法 36只两月龄的APP-PSN转基因鼠,随机分为干预组和对照组,干预组又分高低两个剂量组;12只APP-PSN转基因阴性鼠作为阴性对照组。干预组饲喂添加复合营养素的饲料,其他两组饲喂基础饲料。在干预3个月时,利用Morris水迷宫、穿梭实验、跳台实验三项试验评价各组的认知功能状况。结果各组别的体重变化和摄食量没有统计学差异。干预组水迷宫的潜伏期明显短于对照组,而其跳台实验的潜伏期明显长于对照组,穿梭试验中高低干预组条件反应分别占46.67%和45.00%,对照组条件反应占20.83%。三项行为学实验,干预组与对照组均有统计学差异(P<0.05),对照组和阴性组均有统计学差异(P<0.05),干预组与阴性组均无统计学差异(P>0.05)。结论复合营养素的早期干预能够延缓阿尔茨海默病模型小鼠的认知功能障碍的发生与发展。Objective To observe the early prevention effect of the compound nutrients recipe for cognitive dysfunction of Alzheimer＇ s disease model--APP-PSN transgenic mouse. Methods 36 APP-PSN transgenic mice aged two months randomly were divided into the intervention group supplied with compound recipe in the diet and the control group fed based feed, the former had high dose and low dose, 12 APP-PSN transgenic negative mice aged two months as the negative control were fed based feed. After 3 months＇ intervention, four groups＇ cognitive functions were evaluated using the Morris water maze, active avoidance experiment and jumping stair experiment. Results There was not statistically different between all the four groups for the weight and food intake. Compared with the control group, Morris water maze＇ s incubation period of the intervention group was lower obviously, and jumping stair experiment＇ s incubation period of the intervention group was higher obviously. In the active avoidance experiment, the high and low dose intervention group＇ s conditioned response accounted about 46.67% and 45.00% respectively, and the control group＇ s conditioned response accounted about 20. 83%. The differences of the three behavioral experiments between control group and intervention group had the statistical significance （P 〈 0.05 ）, so the same as between control group and negative control group （P 〈 O. 05 ）. And there was no difference between intervention group and negative control group for the three behavioral experiments. Conclusion The early supplementation with compound nutrition could postpone the occurrence and development of Alzheimer＇ s disease mice model＇ s cognitive dysfunction.

关 键 词：复合营养素 阿尔茨海默病 认知 转基因鼠 

分 类 号：R592[医药卫生—老年医学] R742[医药卫生—内科学]