辛酸钠对奶牛乳腺上皮细胞甘油三酯合成关键酶表达的影响  

Effects of sodium octanoate on key enzymes of triglyceride synthesis in mammary epithelial cells of dairy cows

作　　者：刘莉莉[1] 蒋倩倩[1] 李淑莲[1] 吴涛[1] 丁常宏[1] 赵波[1] 郭艳丽[1] 程玉鹏[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《饲料工业》2014年第1期55-59,共5页Feed Industry

基　　金：黑龙江省教育厅科学技术研究(面上)项目计划[12531638]

摘　　要：以体外培养的奶牛乳腺上皮细胞为模型,研究辛酸钠对细胞甘油三酯(triacylglycerol,TAG)合成能力及其对甘油三酯合成的关键酶甘油-3-磷酸脂酰转移酶(GPAM)、乙酰甘油磷酸脂酰转移酶6(AGPAT6)、磷脂酸磷酸酯酶1(LPIN1)和二酰甘油脂酰转移酶1(DGAT1)表达的影响。采用甘油三酯测试盒检测培养液中甘油三酯浓度;qRT-PCR检测目的基因相对表达丰度;Western blot检测目的蛋白相对表达水平。结果显示,与对照组相比,0.5、1、2 mmol/l的辛酸钠以浓度依赖方式显著降低培养液中甘油三酯的浓度(P<0.05);0.5、1、2 mmol/l的辛酸钠以浓度依赖的方式降低或显著降低GPAM、AGPAT6、DGAT1和LPIN1的mRNA表达和蛋白表达(P<0.05)。本试验结果揭示辛酸钠能抑制奶牛乳腺上皮细胞甘油三酯合成,并对甘油三酯合成关键酶的表达具有抑制作用。This experiment was conducted to investigate the effects of sodium octanoate on triacylglyc- erol （TAG） synthesis and expression level of Glycerol-3-phosphate acyhransferase, l-acylglycerol-3- phosphate O-acyhransferase 6, Lipin 1, and diacylgly-cerol acyhransferase 1 of daiz-y cow mammary epithelial cells （DCMECs） cultured in vitro. In the experiment, TAG contents in medium were detect- ed by TAG quantitation kit, the mRNA expression levels of targeted genes were determined by qRT- PCR, and the protein expression levels of targeted protein were determined by western blotting. The re- suits showed as follows： compared with control group, the contents of TAG in medium were significant- ly decreased in concentration-dependent manner from 0.5 to 2 mmol/l sodium octanoate, the gene ex- pression and protein expression of GPAM, AGPAT6, LPIN1 and DGATI were reduced or significantly reduced by sodium octanoate in concentration-dependent manner from 0.5 to 2 mmol/1. This study sug- gests that sodium octanoate can inhibit TAG accumulation and the expression of key enzymes of tri- glyceride synthesis in DCMECs.

关 键 词：辛酸钠 乳腺上皮细胞 甘油三酯合成 基因表达 蛋白表达 

分 类 号：S823.9[农业科学—畜牧学]