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作 者:赵峰[1] 李碧英[2] 张其文[3] 符伟[3] 张恩华[3]
机构地区:[1]海南广播电视大学,海南海口570228 [2]海南大学应用科技学院,海南海口570228 [3]海南大学农学院,海南海口570228
出 处:《安徽农业科学》2014年第1期24-28,30,共6页Journal of Anhui Agricultural Sciences
基 金:海南省教育厅基金资助项目(编号:Hjkj2012-51)
摘 要:[目的]提取女贞不同种质材料的基因组DNA,并筛选RAPD引物。[方法]采用改良CTAB法提取女贞基因组DNA,并以此种方法提取的女贞基因组DNA为模板,对RAPD引物进行PCR扩增,筛选有效引物。[结果]筛选出21个多态性丰富、条带清晰且重复性好的有效引物,经检测所获得的基因组DNA条带清晰,且OD260/OD280在1.8左右。用筛选出的21个有效引物对126份女贞种质材料进行RAPD-PCR扩增,均可获得带型丰富且清晰可辨的DNA指纹图谱。[结论]该方法为快速和准确地应用RAPD方法分析女贞种质材料的遗传多样性提供了依据。[ Objective ] To extract genomic DNA in different germplasm materials of Ligustrum lucidum and select RAPD primers. [ Method ] Genomic DNA was extracted by improved CTAB method, and with genomic DNA as a template, PCR amplification was conducted on RAPD primers. [ Result ] 21 effective primers with abundant polymorphism, clear bands and good repeatability were obtained. After testing the privet genomic DNA bands were clear, and the OD26o/OD2so was about 1.8. 21 selected effective primers were used in the RAPD-PCR amplification for 126 germplasm materials of L. lucidum. From all of which the abundant and distinct DNA fingerprinting could be obtained. [ Conclusion] The study will provide a reference for the rapid and accurate utilization RAPD method in analyzing genetic diversity of the germplasm materials of L. lucidum.
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