6种中药活性成分对K562/ADM耐药性逆转及P27和P170蛋白表达影响  

Influences of six herbal constituents on protein expressions of P27 and P170 and reversal of K562/ADM cells

作　　者：陈超[1] 蔡天革[2] 张荣华[3] 杨丽[3] 蔡宇[3] 

机构地区：[1]暨南大学附属第一医院,广东广州510630 [2]辽宁大学生命科学学院,辽宁沈阳110036 [3]暨南大学药学院,广东广州510632

出　　处：《中成药》2014年第1期1-4,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助(81072763)

摘　　要：目的观察中药活性成分对白血病K562/ADM耐药细胞逆转及P27和P170蛋白表达的影响,探讨其可能作用机制。方法选用补骨脂素、苦参碱、槲皮素、姜黄素、川芎嗪、大黄酸6种中药单体成分作为研究对象,MTT比色法测定6种中药成分对K562/ADM耐药细胞的增殖抑制作用,高效液相色谱法检测细胞内阿霉素的质量浓度,流式细胞术检测K562/ADM耐药细胞中P27和P170蛋白表达。结果 6种中药活性成分逆转耐药倍数在6.99~10.59之间,K562/ADM耐药细胞中阿霉素积累比率为(11.6±3.7)^(17.7±2.8);对照组P27和P210蛋白的表达率分别为(33.1±2.1)和(6.51±1.33),K562/S和K562/ADM耐药细胞的P27和P210相关蛋白表达率分别在(54.2±3.5)^(72.1±4.2)和(6.21±1.35)^(6.59±1.48)之间。结论 6种中药活性成分具有不同程度的逆转耐药细胞作用,且影响耐药细胞内化疗药物质量浓度;各中药活性成分具有不同程度抑制K562/ADM耐药细胞中P27蛋白表达作用。AIM To observe the influence of six herbal constituents, including psoralen, matrine, quercetin, curcumin igustrazine and rheiM, on leukemia K562/ADM cells reversion and P27 and P170 protein expressions. METHODS MTT colorimetric was used to determine proliferation inhibition of six constituents on K562/ADM cells. HPLC method was used to detect the concentration of adriamycin in the cells, and flow cytometry was applied to detecting P27 and P170 protein expression of K562/ADM cells. RESULTS The reverse ratio of drug resistance fell within 6.99 to 10.59, adriamycin accumulation in K562/ADM cells in multiples of （11.6 _＋ 3.7 ） （17.7 ＋2. 8）, and P210 and P27 protein expression rate of control groups reached （33.1 _＋2. 1） and （6.51 ＋ 2. 1）. K562/S, K562/ADM cells＇ P27 and P210 related protein expression rates were in the range of （54.2 ± 3.5） to （72.1 ±4.2） and （6.21 ±1.35） to （6.59±1.48）. CONCLUSION Six herbal constituents have different abilities to reverse drug resistant cell function, to influence on chemotherapy drug concentration in the cell, and to inhibit P27 protein expression in K562/ADM.

关 键 词：补骨脂素 苦参碱 槲皮素 姜黄素 川芎嗪 大黄酸 K562 ADM耐药细胞 P27 P170 蛋白表达 

分 类 号：R285.5[医药卫生—中药学]