熊胆粉中熊去氧胆酸结晶条件  

Crystallization of ursodeoxycholic acid from bear bile powder

作　　者：陈正[1] 王伯初[1] 祝连彩[1] 刘绍勇 薛东升 

机构地区：[1]重庆大学生物工程学院生物流变学与技术教育部重点实验室,重庆400030 [2]上海凯宝药业股份有限公司,上海201418

出　　处：《中成药》2014年第1期61-65,共5页Chinese Traditional Patent Medicine

基　　金：中央高校基本科研业务费资助(CDJXS11230051)

摘　　要：目的对熊胆粉中熊去氧胆酸的结晶及重结晶过程加以控制,以提高熊胆粉提取物产率及质量。方法通过溶解度实验确定了结晶及重结晶的最佳起始条件,再采用不同的降温方法对该过程进行梯度控温的优化研究。结果以70℃和30℃做为结晶及重结晶的始末温度;筛选出最佳的结晶控温条件为控温8 h,前5 h每1 h降温2℃,后3 h每1 h降温10℃,重结晶工艺控温条件与结晶控温条件一致。应用该结晶工艺,与原工艺相比,熊胆粉提取物产率提高2.38%,转化率提高12.35%,熊去氧胆酸量提高5.83%,鹅去氧胆酸量基本保持不变。结论本研究所建立的结晶工艺,可显著提高熊胆粉中熊去氧胆酸的产率和质量。AIM To control the crystallization and recrystallization process in ursodeoxycholic acid extracted from bear bile powder, as well as their improvement in yield and quality. METHODS The optimal initial condi- tions of crystallization and recrystallization were obtained by the solubility experiments. The extraction from bear bile powder process was improved by different methods of gradient temperature controlling. RESULTS The initial and final temperature of crystallization and recrystallization was 70 ~C and 30 ~C , respectively. It was screened out that the best condition for the crystallization and recrystallization was 8 h. In the first 5 hours, the temperature de- creased at the rate of 2 ＂C per hour. In the following 3 hours, the temperature decreased at the rate of 10 ~C per hour instead. Comparing with the original process, the extract rate of bear bile powder products increased by 2.38% . The conversion rate increased by 12. 35% . The content of ursodeoxycholic acid increased by 5.83% , and cbenodeoxycholic acid content kept steady. CONCLUSION The established crystallization process can significantly improve the yield and quality of bear bile powder extract.

关 键 词：熊去氧胆酸 梯度降温 结晶过程 熊胆粉 

分 类 号：R284.2[医药卫生—中药学]