多波长RP-HPLC法同时测定肥儿丸中木香烃内酯、去氢木香内酯、去氢二异丁香酚  

Simultaneous determination of costunolide,dehydrocostus lactone,dehydrodiisoeugenol in Feier Pills by multiwavelength RP-HPLC

作　　者：刘晓哲[1] 

机构地区：[1]河南省南阳市药品检验所,河南南阳473059

出　　处：《中成药》2014年第1期98-101,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立同时测定肥儿丸（煨肉豆蔻、木香等）中木香烃内酯、去氢木香内酯、去氢二异丁香酚的方法。方法采用HPLC法，C18色谱柱；以乙腈-水（70：30）为流动相；体积流量1．0mL／min；检测波长225nm（木香烃内酯、去氢木香内酯），274nm（去氢二异丁香酚）。结果根据回归方程，3种成分线性范围分别为木香烃内酯10．834—216．68μg／mL，r^2=0．9996；去氢木香内酯9．506～190．12μg/mL，r^2=0．9994；去氢二异丁香酚5．782—115．64μg/mL，r^2=0．9989。平均回收率分别为木香烃内酯98．4％，RSD为0．66％（n=6）；去氢木香内酯99．1％，RSD为1．16％（n：6）；去氢二异丁香酚98．1％，RSD为1．25％（n=6）。结论该方法经济适用，操作简便、准确，重复性好，可用于肥儿丸的质量控制。AIM To establish a method for simultaneously determining the levels of costunolide, dehydrocostus lactone, and dehydrodiisoeugenol in Feier Pills （Myristicae Semen, Aucklandiae Radix, etc. ）. METH- ODS The quantitative analysis was carried out on a column of Kromasil C18 by HPLC, by using a mobile phase of acetonitrilewater under a flow rate of 1.0 mL/min. 225 nm for costunolide and dehydrocostus lactone, 274 nm for dehydrodiisoeugenol as the detection wavelength. RESULTS The linear ranges of eostunolide, dehydro- costus laetone, and dehydrodiisoeugenol fell within the ranges of 10. 834-216.68 μg/mL, 9. 506-190.12μg/mL, 5. 782-115.64 p.g/mL, respectively. The three components showed good linear correlations （r^2 = 0. 999 6, 0. 999 4, 0. 998 9）. The recoveries were 98.4% for costunolide, 99.1% for dehydrocostus laetone, 98. 1% for dehydrodiisoeugenol, respectively. The relative standard deviations were 0.66% , 1. 16% , and 1.25% , respectively. CONCLUSION The method is simple, economical, accurate, reproducible and con- venient for the quality control over Feier Pills.

关 键 词：高效液相色谱 肥儿丸 木香烃内酯 去氢木香内酯 去氢二异丁香酚 

分 类 号：R927.2[医药卫生—药学]