健那绿-硫酸软骨素体系的共振瑞利散射光谱及其分析应用  

Resonance Rayleigh Scattering Spectra of Janus Green-Chondroitin Sulfate System and Its Analytical Application

作　　者：韩权[1] 田丽[2] 郝甜甜[1] 翟云会[1] 霍燕燕[1] 

机构地区：[1]西安文理学院化学与化学工程学院,陕西西安710065 [2]陕西省疾病预防控制中心理化检验中心,陕西西安710054

出　　处：《中国医药工业杂志》2014年第1期79-82,共4页Chinese Journal of Pharmaceuticals

基　　金：陕西省自然科学基础研究计划项目(2010JM2018);西安市科技计划项目(CXY1134WL38)

摘　　要：在pH8．5～9．5的Britton—Robinson缓冲溶液中，健那绿与硫酸软骨素作用形成结合产物时导致溶液共振瑞利散射（RRS）显著增强并产生新的RRS光谱，其最大散射峰位于326nnq处，另在407nm和560nn3处有两个强度较小的散射峰。硫酸软骨素在0．1～5．0μg／ml浓度范围内与RRS强度的线性关系良好。其最低检测限为25．3ng／ml。In pH 8.5 - 9.5 Britton-Robinson buffer media, a compound complex was formed between janus green and chondroitin sulfate, leading to a great enhancement of the intensity of resonance Rayleigh scattering （RRS） and giving a new RRS spectrum. The maximum scattering peak was located at 326 nm and two relatively weaker peaks were located at 407 nm and 560 nm. It was also found that the intensity of RRS was directly proportional to the concentration of chondroitin sulfate in the range of 0.1 - 5.0 μg/ml. The low limit of detection was 25.3 ng/ml.

关 键 词：共振瑞利散射 健那绿 硫酸软骨素 测定 

分 类 号：O657.3[理学—分析化学]