γ-聚谷氨酸胆甾醇基衍生物自组装纳米胶束的制备与表征  

Preparation and Characterization of Self-Assembly Nanoparticles of Cholesterol-Bearingγ-Polyglutamic Acid Derivatives

作　　者：李睿[1] 阮文辉 姚俊[3] 陈宽婷[3] 魏钦俊[3] 鲁雅洁[3] 曹新[3] 

机构地区：[1]山西中医学院中药学院,山西太原030024 [2]山西省医药与生命科学学院,山西太原030006 [3]南京医科大学基础医学院生物技术系,江苏南京210029

出　　处：《高分子材料科学与工程》2014年第1期11-14,共4页Polymer Materials Science & Engineering

基　　金：江苏高校优势学科建设工程资助项目;山西省科技攻关项目(20110321081-01,20120313016-6);山西省青年基金资助项目(2013021034-4)

摘　　要：对水溶性的γ-聚谷氨酸(γ-PGA)进行接枝改性,合成了两亲性胆甾醇基γ-PGA衍生物;通过红外光谱(FT-IR)及核磁共振(1H-NMR)对其结构进行了表征和确证;并采用超声探头法制备得胆甾醇基γ-PGA自组装胶束。在中性介质条件下,形成的胶束在透射电镜下观察呈规则球状,平均粒径为(299.6±5.4)nm,粒径的多分散系数(PDI)为0.17。实验表明,两亲性胆甾醇基γ-PGA自组装体的形成是分子内分子间疏水作用力、分子带电情况和分子柔性变化等协同作用的结果,而介质pH是重要的影响因素,其形成的核壳结构可作为疏水药物和大分子药物的载体。An amphiphilic graft copolymer composed of γ-polyglutamic acid （7-PGA） as the hydrophilic backbone and cholesterol as the hydrophobic segment was synthesized. The structure of cholesterol-bearing γ-PGA （7-PGA- graft-CH） was studied by FT-IR and ^1H-NMR. Amphiphilic γ-PGA-graft-CH was able to form self-assembly nanoparticles composed of an inner hydrophobic core and an outer shell of hydrophilic groups. Self-assembly nanoparticles were formed by the ultrasonic probe method. The average diameter of obtained nanoparticles is （299.6 ＋ 5.4）nm at neutral pH, particle size distribution （PDI=0.17） is narrow. The experimental results also show that the formation of self-assembly nanoparticles was significantly affected by pH value of the medium as a result of the comprehensive influence of molecular charge and flexibility of γ-PGA-Graft-CH, as well as the hydrophobic interaction. The self-assembly nanoparticles of γ-PGA-Graft-CH can be used as an effective carrier of some hydrophilic or macromolecular drugs

关 键 词：Γ-聚谷氨酸 两亲性聚合物 自组装 纳米胶束 

分 类 号：TB383[一般工业技术—材料科学与工程]