白藜芦醇对大鼠应激性胃溃疡的保护作用  

Protective effect of resveratrol on stress-induced gastric ulcer in rats

作　　者：问慧娟[1] 李玉巧[1] 崔玉英[1] 韩璇[1] 

机构地区：[1]河北大学基础医学院,河北保定071000

出　　处：《中国医院药学杂志》2014年第1期15-18,共4页Chinese Journal of Hospital Pharmacy

基　　金：河北省中医药类科研计划项目(编号:2012134);河北省科学技术研究与发展计划项目(编号:11276103D-7);河北省卫生厅重点科技研究计划项目(编号:20100141)

摘　　要：目的:探讨白藜芦醇对大鼠应激性胃溃疡的影响及其可能机制。方法:30只Wistar大鼠随机分为对照组,模型组,白藜芦醇低、中、高剂量组,采用水浸-束缚应激(WRS)大鼠模型,计算各组胃溃疡指数,测定胃黏膜血流量(GMBF)、胃黏膜组织诱导型一氧化氮合酶(iNOS)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与模型组比较,白藜芦醇各组能明显降低WRS大鼠的胃溃疡指数,以中剂量组作用显著(P<0.01);显著提高胃黏膜血流量(P<0.01),降低胃黏膜组织iNOS活性和MDA水平(P<0.05),提高SOD活性(P<0.01)。结论:白藜芦醇对应激性溃疡具有显著的预防作用,其机制可能与增加胃黏膜血流量,抗氧化和抑制iNOS有关。OBJECTIVE To study the protective effects of resveratrol on the stress-induced gastric ulcer in rats and its mechanism of action.METHODS 30 Wistar rats were randomly divided into five experimental groups：control group,model group, low dose resveratrol group,medium dose resveratrol group and high dose resveratrol group.By using water immersion and restraint stress（WRS）rat model,we calculated the index of each group of gastric ulcer and assessed the gastric mucosal blood flow（GMBF）,inducible nitric oxide synthase（iNOS）,the content of malondialdehyde（MDA）,the activity of superoxide dismutase（SOD）.RESULTS Compared with the model group,the resveratrol group could significantly reduce the gastric ulcer index of WRS rats,medium dose resveratrol group showed the best results（P0.01）.It could significantly increase the gastric mucosal blood flow（P0.01）,obviously reduce the activity of iNOS in gastric mucosal tissue and lower its MDA level（P 0.05）,increase the SOD activity（P0.01）.CONCLUSION These findings collectively suggest that resveratrol has a significant protective effect on stress-induced ulcers,the mechanism of which is related to the increase of gastric mucosal blood,oxidation resistance and inhibition of iNOS.

关 键 词：白藜芦醇 应激 胃溃疡 胃黏膜血流量 SOD INOS 

分 类 号：R285.5[医药卫生—中药学]