人类免疫缺陷病毒B亚型包膜糖蛋白V4区变异与CD4^＋T细胞数相关性分析  

Correlation analysis between the variation of fourth variable region of subtype B human immunodeficiency virus type 1 envelope and patient CD4^ ＋T cell counts

作　　者：李妍[1] 李迪[1] 杨丹[1] 王甲业[1] 庄敏[1] 凌虹[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,150081

出　　处：《国际免疫学杂志》2014年第1期74-77,共4页International Journal of Immunology

基　　金：黑龙江省教育厅基金资助（12511184）

摘　　要：目的探讨人类免疫缺陷病毒感染者包膜蛋白V4区变异与CD4^＋T细胞数之间的关系。方法本研究从Los Alamos HIV序列数据库中选取369条HIV-1B亚型且已知CD4^＋T细胞数的V4区氨基酸序列。分析CD4^＋T细胞数与V4区氨基酸序列长度、潜在糖基化位点（PNGS）及净电荷数的相关性。V4区氨基酸序列按CD4^＋T细胞数高低分成两组：CD4^＋T细胞数〈200个／μL组（n=216）和CD4^＋T细胞数≥200个／μL组（n=153），比较两组V4区氨基酸的差异。结果CD4^＋T细胞数与V4区氨基酸序列长度及PNGS数正相关（r=0．248，0．136，P均〈0．01），而与V4区净电荷数负相关（r=-0．154，P〈0．01）。CD4^＋T细胞数〈200个／μL与CD4^＋T细胞数〉200个／μL组相比，V4区氨基酸序列长度较短（P〈0．01）、PNGS数较少（P〈0．05）。但CD4^＋T细胞数高低两组V4区净电荷数之间无统计学意义（P=0．17）。结论V4区变异和HIV-1感染的病程具有相关性，随着CD4^＋T细胞数的降低，V4区序列变短、PNGS数减少。Objective To characterize the correlation between the variation of V4 sequence and CD4^ ＋ T cell counts. Methods 369 V4 sequences with CD4 ^＋ T cell counts known were downloaded from the Los Alamos HIV Sequence Database. Correlations between CD4^ ＋ T cell counts and length, the number of PNGS, net charge were assessed for univariate comparisons. Furthermore, All the sequences were classified into high CD4 ^＋ T cell counts （〉^＋200/p^＋L） group and low CD4 ＋ T cell counts group （ 〈 200/μL）. No-parametric test was used to discover the different between the two groups. Results There were significant positive correlations between the CD4 ^＋ T cell counts and length, PNGS （r = 0. 248,0. 136,P 〈 0.01 ）. However, the results showed a negative association between the C D4 ^＋ T cell counts and net charge（r = -0. 154,P 〈 0.01 ）. Moreover, there were less number of amino acids （P 〈 0.01 ） and PNGS （P 〈 0.05 ） of V4 sequences with CD4 ^＋ T cell counts 〈 200 cells/μL. Conclusion The length and PNGS of V4 were decreased with the decline of CD4 ^＋ T cell counts, which indicated that there was a correlation between the variation of V4 and CD4 ^＋ T cell counts.

关 键 词：人类免疫缺陷病毒-1 包膜糖蛋白 V4区 CD4^+T细胞数 

分 类 号：R512.91[医药卫生—内科学]