药物抑制乙肝病毒血清标志物的实验研究  

Experimental Study of Screening Test of Anti-HBV Drugs

作　　者：王新[1] 张京秀[1] 倪苑[1] 

机构地区：[1]首都医科大学附属北京同仁医院,100730

出　　处：《天津医药》2001年第2期97-99,共3页Tianjin Medical Journal

摘　　要：目的:通过药物抑制乙肝病毒血清标志物(HBV-M)的实验,观察药物抑制HBV的作用。方法:先将药物做初筛试验,再对选择出的药物进行酶抑制试验和对照试验。用ELISA检测各组的HBV-M光密度(OD)值。结果:经初步筛选,可见84消毒剂和藿香正气水能够使HBsAg和HBeAg的OD值明显降低。藿香正气水可使二者OD值低于阴性界限值(Cutoff)。酶抑制试验显示其对ELISA无明显干扰。对照试验中,藿香正气水抑制HBsAg的OD值曲线也表明其能有效抑制HBV-M的抗原性。结论:药物抑制HBV-M的实验研究。Objective:To investigate the effect of anti-HBV drugs using the experiment of anti-HBV-M. Methods:Drugs were selected with screening test,and were deteced with inhibition of HRP and control test. The optical density (OD) values of HBV viral marks were determined with ELISA. Results:The OD value of HBsAg and HBeAg in disinfectant 84 and Huo Xiang Zheng Qi Shui treatment groups decreased, and was lower than the cutoff in Huo Xiang Zheng Qi Shui treatment group. The drug did not show any obvious interference with ELISA in the inhibition test of HRP.The curve of OD value from Huo Xiang Zheng Qi Shui inhibiting HBsAg showed that Huo Xiang Zheng Qi Shui can effectively inhibit the antigen of HBV-M by control test.Conclusion:The study of screening test of anti-HBV drugs can provide the evidence of screening anti-HBV.

关 键 词：乙型肝炎病毒 血清标志物 药物筛选 乙型肝炎 治疗 预防 

分 类 号：R978.7[医药卫生—药品] R512.620.5[医药卫生—药学]