川芎对庆大霉素致聋豚鼠耳蜗氧自由基生成的影响  

Effects of Ligustrazine(TMP)on Acute Drug-induced Free Radical Formation in Guinea Pig Cochlear

作　　者：王永华[1] 王一鸣[1] 沈晓丽[1] 韩明训[1] 

机构地区：[1]浙江中医药大学耳聋康复研究所,杭州310053

出　　处：《听力学及言语疾病杂志》2014年第1期73-75,共3页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金面上项目(81173309)

摘　　要：目的研究川芎注射液(tetramethylpyrazine,TMP)对庆大霉素致聋豚鼠耳蜗内氧自由基生成的影响,探讨TMP对急性药物性聋的干预作用及机制。方法选择正常的健康豚鼠60只随机分成正常对照组(10只)、TMP组(10只)、庆大霉素组(GM组,20只)、GM+TMP组(20只)。GM组每日腹腔注射硫酸庆大霉素120mg/kg,TMP组每日腹腔注射盐酸川芎嗪40mg/kg,GM+TMP组同时注射硫酸庆大霉素和盐酸川芎嗪,剂量同GM组和TMP组;正常对照组则每日腹腔注射等量生理盐水,均连续用药10天,每日一次。各组动物于用药前及停药后第1天分别行ABR测试,停药后第1天测试ABR后用羟胺法和硫代巴比妥法检测各组豚鼠耳蜗组织中超氧化物歧化酶(SOD1、SOD2、T-SOD)活力和丙二醛(MDA)含量。结果用药10天后GM组ABR反应阈明显升高,与用药前、正常对照组及GM+TMP组比较差异有统计学意义(P<0.01),TMP组用药前后ABR反应阈差异无统计学意义(P>0.05)。GM组耳蜗组织中MDA含量显著升高,与正常对照组、GM+TMP组、TMP组比较差异有统计学意义(P<0.05);GM+TMP组、TMP组与正常对照组之间耳蜗MDA含量比较差异无统计学意义(P>0.05)。GM+TMP组耳蜗组织中T-SOD活性明显高于GM组,差异有统计学意义(P<0.05),GM组其活性低于正常组(P<0.05)。GM组耳蜗组织中SOD1活性明显下降,且与正常对照组比较差异有统计学意义(P<0.05),GM+TMP组耳蜗组织中SOD1、SOD2活性明显高于GM组(P<0.05)。结论 TMP可通过提高耳蜗组织内SOD的活力,以防止脂质的过氧化,从而减轻庆大霉素对耳蜗的早期损伤。Objective To explore the effects of ligustrazine （TMP） on acute drug-induced free radical for-mation in guinea pig cochlear,and to explore possible mechanisms of TMP on gentamyein（GM）-induced ototoxici-ty. Methods Sixty guinea pigs were randomly divided into four groups, control group, GM group, TMP group and TMP＋GM group. GM group were injected with GM 120 mg/kg per day. TMP group were injected with TMP 40 mg/kg per day. TMP＋GM group were injected with GM and TMP at the same dosage. Control group were injected with normal saline. All groups were injected for consecutive 10 days. Before injection and one day after the last injec-tion, ABR thresholds were measured. Biochemical assays of Superoxide Dismutase （SOD1, SOD2,T-SOD） activity and malondialdehyde（MDA） content in guinea pig cochlear were detected by TBA. Results After the injection, ABR thresholds in GM group were significantly increased compared with those of in control group（P〈0.01）, with significant difference between GM-FTMP and GM group（P〈0.01）. Before and after the experiment, ABR thresholds in control group were almost unchanged（P〉0.05）. SOD activity was significantly decreased while MDA content was increased in cochlear tissues after GM injection（P〈0.05）. Co-treatment with TMP evidently enhanced SOD activity and decreased MDA content（P〈0.05）. Oonclusion TMP may enhance SOD activity and prevent lipid per-oxidation,thus alleviate GM ototoxicity, and improve auditory function.

关 键 词：川芎 庆大霉素 丙二醛 耳蜗 

分 类 号：R764.5[医药卫生—耳鼻咽喉科]