Folin-Denis分光光度法测定五倍子中单宁酸的含量  

Determination of Tannic Acid Content in Gallnut by Folin-Denis Spectrophotometry

作　　者：耿娜娜[1] 李学英[1] 顾丁[1] 刘林 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,贵州遵义563099 [2]遵义林源医药化工有限责任公司,贵州遵义563099

出　　处：《安徽农业科学》2013年第29期11848-11850,11915,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州省教育厅特色药用资源研发创新团队项目([2013]15)

摘　　要：[目的]探索Folin-Denis可见分光光度法精确快速测定五倍子中单宁酸含量的可行性。[方法]用水温浸提法消化五倍子后，其提取液中的单宁酸与Folin-Denis试剂反应形成稳定的蓝色配合物，通过测定该反应体系的吸光度来确定单宁酸的含量，同时验证最佳的检测条件。[结果]单宁酸浓度在0．002-0．010mg／ml范围内，吸光度和单宁酸浓度具有良好的线性关系，符合回归方程A=44．31c＋0．008，R。=O．999O，加标回收率为97．8％～101．8％，相对标准偏差（RSD）〈0．5％，单宁酸平均含量为82．O％。[结论]利用Folin-Denis分光光度法测定五倍子中单宁酸的含量，操作简便、快速、精密度高、重现性好、加标回收率较高。[ Objective] To explore the feasibility of using Folin-Denis visible spectrophotometry to detect accurately and qmckty the content oI tannic acid in gallnut. [ Method] Gallnut was digested with water extraction method, then tannic acid in the extract and Folin-Denis can react to form a stable blue complex. According to measuring the absorbance of the color reaction system, the content of tannic acid was determined. At the same time, the best testing conditions were proved. [ Result] There was a linear relationship between absorbance and concentration of tannic acid, when the concentration of tannic acid was in the range of 0.002 -0.010 mg/ml. The regression equation was A = 44. 31c ＋ 0. 008 ,R2 -- O. 999 0, and that the recovery rate was ranged from 97.8% to 101.8%, with an RSD （ relative standard deviation） less than 0.5%, and that the average content of tannic acid was 82.0%. [ Conclusion] Folin-Denis spectrophotometer could be a rapid method to detect the content of gall- nut tannic acid with its simplicity, convenience, high precision, reproducibility, and high recovery.

关 键 词：Folin—Denis试剂 分光光度法 五倍子 单宁酸 测定 

分 类 号：TQ947[化学工程]