灌胃凤凰衣水解物对膝骨关节炎模型大鼠影响的实验研究  

Effect of Hydrolyzed Eggshell Membrane on Knee Osteoarthritis in Rats

作　　者：李奕修[1] 余添赐[1] 魏迎辰[1] 邱慈鑫 杨树华[1] 李楠[1] 

机构地区：[1]福建中医药大学骨伤学院省部共建教育部重点实验室,福州350122

出　　处：《中国中医骨伤科杂志》2014年第1期8-11,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:观察灌胃凤凰衣水解物对膝骨关节炎大鼠膝关节的影响,进而考察凤凰衣用于防治OA的合理性及可行性。方法:灌胃凤凰衣水解物及奥泰灵胶囊干预膝骨关节炎大鼠,观察软骨形态及软骨中Collagen-Ⅱ、MMP-3及蛋白多糖的指标变化,以及血清和关节液中IL-6,IL-1β,TNF-α的指标变化。结果:1.各用药组对降低膝骨关节炎大鼠血清IL-1β、IL-6、TNF-α表达,关节液中TNF-α的表达以及软骨中MMP-3表达,升高Collagen-Ⅱ的表达看,两用药组未见明显差异(P>0.05),但均优于模型组(P<0.01);2.在提高蛋白多糖含量及降低关节液中IL-6的表达方面,灌胃凤凰衣组优于灌胃奥泰灵组(P<0.01);3.在降低关节液中IL-1β的表达方面,灌胃奥泰灵组优于灌胃凤凰衣组(P<0.01)。结论:1.灌胃凤凰衣水解物能降低膝骨关节炎大鼠的炎症因子表达,提高关节软骨中蛋白多糖和Collagen-Ⅱ含量;2.凤凰衣中含有丰富的可以合成软骨基质中蛋白多糖的前体物质,是其改善OA指标的机制之一。Objective：To observe the influence of intragastric administration with hydrolyzed eggshell membrane on knee os- teoarthritis in rats. Methods：Rats with knee osteoarthritis were intragastrieally administered hydrolyzed eggshell membrane or glucosamine hydrochloride. Cartilage shape, proteoglycan, collagen-Ⅱ and MMP-3 in cartilage were observed. The levels of IL-6, IL-1β and TNF-a in serum and joint fluid were measured. Results： 1. The serum levels of IL-1β, IL-6 and TNF-α, synovial fluid TNF-a level as well as MMP-3 in cartilage were decreased in both treatment groups, with no signifi- cant difference in collagen-Ⅱ （P〈0.05）. 2. Proteoglyean in hydrolyzed eggshell membrane group was increased much more than that in glucosamine hydrochloride group. 3. IL-1β in synovial fluid in glucosamine hydrochloride group was de- creased much better than that in hydrolyzed eggshell membrane group （P〈0.01）. Conclusion： 1. Hydrolyzed eggshell membrane can reduce inflammatory factor expression of rats with knee osteoarthritis and increase proteoglycan and Colla- gen-Ⅱ content in articular cartilage. 2. Eggshell membrane contains abundant precusor substance that forms proteoglycan in cartilage matrix, which is one of the mechanisms in improvement of osteoarthritis by eggshell.

关 键 词：凤凰衣 骨关节炎 关节腔注射 蛋白多糖 

分 类 号：R33[医药卫生—人体生理学]