自由活动大鼠睡眠呼吸监测技术改进  

Improved methods for monitoring sleep state and respiratory rhythm in freely moving rats

作　　者：王奇敏[1] 董慧[1] 张成[1] 张永鹤[2] 马靖[1] 王广发[1] 

机构地区：[1]北京大学第一医院呼吸与危重症医学科,北京100034 [2]北京大学基础医学院药理学系,北京100083

出　　处：《中国应用生理学杂志》2014年第1期27-30,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金(30900646)

摘　　要：目的:改进大鼠睡眠呼吸监测技术,解决既往模型中咬线、脱落、信号不稳定问题。方法:改进监测电极、信号接入线路及动物手术。结果:改进后手术时间缩短,创面暴露时间减少,大鼠术后易恢复。且电极稳固性好,脑电和肌电信号更稳定清晰,信号接入线路传导性好,耐用。实现连续长时间监测,成功率高。便于根据信号进行不同清醒-睡眠状态分期,并判断大鼠睡眠呼吸暂停情况。结论:改进后的操作技术操作更简单、信号更稳定,是一项更加实用可靠的小动物睡眠呼吸监测技术。Objective： To improve the method for monitoring sleep state and respiratory rhythm of SD rats, providing a solution for rats＇ chewing on the wires, signal loss and instability problems in the animal model of sleep apnea syndrome （SAS）. Methods： We improved moni- toring electrodes of both electrocorticogram （ECoG） and electromyogram （EMG）, signal circuit and animal operation. Retmlla： Operation time was shortened and wound exposure time was reduced, which made it easier for postoperative recovery. The ECoG and EMG signals were more stable with sharp image, and signal circuit lines had better conductivity and material durability, achieving continuous monitoring for a long time and high success rate. We could precisely distinguish the sleep wake state and the sleep apnea events in rats according to these signals. Conclusion： The improved method is more reliable and practical to test the small animal model of SAS, and is more easily to operate and the signals axe more stable.

关 键 词：睡眠 监测 大鼠 

分 类 号：R338.2[医药卫生—人体生理学] R563.9[医药卫生—基础医学]