高湿环境对大鼠免疫功能的影响  

Effect of high humidity environment on immune function in rats

作　　者：郭鑫[1] 李昆[1] 王超[1] 李薇[1] 杨芸[1] 宋富强[1] 呼永河[1] 

机构地区：[1]成都军区总医院中心实验室,四川成都610083

出　　处：《中国应用生理学杂志》2014年第1期89-92,共4页Chinese Journal of Applied Physiology

基　　金：全军医学科技"十二五"重点项目(BWS11J067)

摘　　要：目的:观察高湿环境下大鼠免疫功能的变化,并探讨其意义,为进一步研究相关影响机制奠定基础。方法:将30只SD大鼠随机分为3组(n=10):分别为常温常湿组、湿化20 d组和40 d组。湿化模型组用人工环境气候箱模拟高湿环境,在90%的湿度条件下进行处理,并于第20天、40天分别处死,收集外周血和脾脏,用流式细胞术检测大鼠外周血和脾脏的T淋巴细胞的百分率。结果:湿化20 d大鼠外周血CD3+%为52.91±6.27,CD4+%为37.80±4.11,CD8+%为14.85±3.73,CD4+/CD8+为2.72±0.82,除CD3+%外与常温常湿组均有显著性差异(P<0.05),而40 d组外周血T淋巴细胞亚群数据均无统计学差异。湿化各组脾脏T淋巴细胞的百分率与常温常湿组差异不大,20 d组CD8+%为6.23±2.87,有显著性差异(P<0.05)。血清检测结果显示,高湿组IgG、IgA、IgM均未见显著变化,而20 d组C3显著升高。结论:在高湿环境下,初期大鼠的免疫自稳状态发生变化,免疫功能升高,随着时间的推移,机体通过自我的调整,可能逐渐习服,免疫功能逐渐降低到正常水平。Objective： To investigate effects of the variation of immune function in high humidity environment in different time, and lay a foundation for further study of the related mechanism. Method： Thirty SD rots were divided into 3 groups （ n = 10）： 20 day group,40 day group in 90% relative humidity chamber and control group in normal relative humidity. Peripheral blood and spleens were collected to detect the levels of T lymphocyte subsets by Flow Cytometery. Results： In peripheral blood of the 20 day group rats, the CD3＋ %, CD4＋ %, CD8 ~ % and CD4 ~/CD8 ~ were 52.91 ＋ 6.27,37.80 ＋ 4.11,14.85 ~： 3.73 and 2.72 ＋ 0.82 separately. Expect CD3 ~ %, they all had sig- nificant differences （ P 〈 0.05）. In addition, the data of the 40 day group rats showed no diversity in statistics. In spleen, CD8 ~ % of the 20 day group rots was 6.23 ＋ 2.87 with significant differences （ P 〈 0.05） and IgG, IgA and IgM did not change a lot in blood serum of the high humidity groups except C3 of the 20 days group （ P 〈 0.05）. Conclusion： In high humidity environment, the immune function of the rats in- creased in the initial stage. As time went on, the immune function gradually went to normal level through the self adjustment.

关 键 词：高湿模型 T淋巴细胞亚群 流式细胞术 

分 类 号：R392[医药卫生—免疫学]