稻曲病菌UvHog1基因的克隆及表达分析  

Cloning and Expression Analysis of UvHog1 Gene in Ustilaginoidea virens

作　　者：饶玉春[1,2] 丁正中[1,3] 陈析丰[1] 曾大力[2] 马伯军[1] 顾志敏[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [3]金华市艾青中学,浙江金华321015

出　　处：《中国水稻科学》2014年第1期9-14,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31240079;30800677;31201183);浙江省自然科学基金资助项目(LY12C06001);浙江省重中之重学科开放基金资助项目

摘　　要：稻曲病由稻曲病菌引起,是水稻穗部的重要病害。利用同源克隆和RACE技术,根据丝状真菌相对保守的氨基酸序列设计简并引物,从稻曲病菌中分离了丝裂原活化蛋白激酶(MAPK)编码基因的全长cDNA序列,命名为UvHog1。UvHog1含有MAPK保守的蛋白激酶激活域(TGY),序列与稻瘟病菌MgOsm1(AAF09475.1)、球孢白僵菌BbHog1(AAS77871.1)、烟曲霉AfOsm1(XP752664.1)和隐球酵母CrHog1(AAM26267.1)等MAPK高度同源,相似性分别为95%、93%、88%和81%。系统聚类结果表明,UvHog1与酵母Hog1MAPK同源。在盐胁迫条件下,UvHog1的相对表达量明显降低,表明UvHog1参与了对盐胁迫的信号响应。Rice false smut, caused by Ustilaginoidea virens, is an important disease in rice panicle. Degenerate PCR primers were designed according to the conserved amino acid sequence of several filamentous fungus mitogen activated protein kinase（MAPK） homologous to yeast Hogl MAPK, and partial DNA fragment encoding a MAPK was amplified from U. virens through PCR. Then, a whole DNA sequence encoding the MAPK, designated as UvHogl, was obtained from U. virens by extending upstream and downstream sequence of the amplified fragment using RACE method. And its amino acid sequence shared 95%, 93%, 88% and 81% identity to MgOsml （AAF09475.1） from Magnaporthe grisea, BbHogl （AAS77871.1） from Beauveria bassiana, AfOsml （ XP752664.1 ） from Aspergillus jurnigatus and CrHogl （AAM26267.1） from Cryptococcus ne@rmans var. neoformans, respectively. Phylogenetic clustering suggested that UvHogl is homologous to yeast Hog1 MAPK. The results of the decreased transcript levels of UvHogl by salinity suggest that UvHogl may be involved in the specifically responses to salt stresses.

关 键 词：稻曲病菌 Hog1 克隆 表达分析 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]