分子标记辅助选择改良宁恢8号的条纹叶枯病抗性  

Improving Resistance to Rice Stripe Disease of Restorer Line Ninghui 8 by Molecular Marker-Assistant Selection

作　　者：姚姝[1] 陈涛[1] 张亚东[1] 朱镇[1] 赵庆勇[1] 周丽慧[1] 赵凌[1] 李余生[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心/江苏省优质水稻工程技术研究中心,南京210014

出　　处：《中国水稻科学》2014年第1期85-91,共7页Chinese Journal of Rice Science

基　　金：江苏省科技支撑计划资助项目(BE2009303-1);国家863计划资助项目(2011AA10A101)

摘　　要：为改良宁恢8号的条纹叶枯病抗性,以含条纹叶枯病抗性基因Stv-bi的品种关东194为供体,通过杂交、回交,利用与条纹叶枯病抗性基因Stv-bi和恢复基因Rf1a共分离的分子标记进行辅助选择,将条纹叶枯病抗性基因Stv-bi导入宁恢8号,结合田间抗性和性状鉴定,至BC3F3育成10个条纹叶枯病抗性显著提高、农艺性状优良的改良系。选择其中4个综合性状较优的株系分别与不育系A25、A19和A70进行配组鉴定。结果表明,改良系-2所配组合的综合性状明显优于对照宁恢8号的相应组合。A molecular marker assisted selection （MAS） strategy was used to improve the resistance to rice stripe disease of Ninghui 8, the restorer line of japonica hybrid 86 you 8, by advanced backcross with Ninghui 8 as the recurrent parent and Kanto 194 as the donor of the rice stripe disease resistant gene. Markers tightly linked with Stv-b~ and Rfla genes were used for selecting the two genes of the ability to recovery the sterility and to resistant the stripe disease, respectively. After identification of the rice stripe disease resistance and investigation of some agronomic traits, 10 resistance improved lines with similar characteristics as Ninghui 8 were developed in BCa Fa generation, and 4 lines with excellent characteristics were selected to cross with male-sterile lines A25, A19 and A70, respectively. The results indicated that comprehensive characters of hybrid combinations from improved line-2 were significantly better than those from Ninghui 8.

关 键 词：杂交粳稻 恢复系 条纹叶枯病抗性 分子标记辅助育种 

分 类 号：S511[农业科学—作物学]